玉米大斑病菌特异性毒素组分的分离与纯化  

Isolation and purification of specific toxin fractions produced by Exserohilum turcicum

作　　者：张利辉[1] 刘云惠[1] 董金皋[1] 李正平[1] 

机构地区：[1]河北农业大学真菌毒素实验室,保定071001

出　　处：《植物病理学报》2003年第1期67-71,共5页Acta Phytopathologica Sinica

基　　金：国家教育部骨干教师计划项目;河北省自然基金资助项目 (30 2 31 8)

摘　　要：玉米大斑病菌的 2号小种菌株用改良Fries培养液进行体外培养 ,培养滤液经 - 40℃冷冻干燥 ,加等体积甲醇去除沉淀后用乙酸乙酯提取 ,粗提物经HPLCC18柱可以分离出 8个组分 (峰 3～ 10 )。经生物测定发现 ,7号峰和 10号峰的纯品对玉米叶片有明显的毒性 ,其中 7号峰对带有Ht1基因的玉米表现出了一定的特异性。制备所得毒性组分分别进行红外光谱扫描 ,发现 2个毒性组分的红外吸收光谱基本一致 ,吸收峰形状基本相同 ,只是其波数稍有变动。Crude toxins were extracted from culture filtrate of Exserohilum turcicum race 2 in modified Fries medium after freeze-dried at -40℃ and precipitated with methanol. Eight fractions (peak 3-10) were isolated from the crude toxins by means of HPLC analysis with C 18 column. Peak 7 and 10 had distinct toxicity to the corn leaves and peak 7 had special toxicity to the corn with Ht1 resistant gene. Both the toxic fractions were scanned by infrared spectrum. It was shown that the two toxic fractions (peak 7 and 10) had analogous spectrum,similar shape of peaks,but a little difference in the wavenumbers.

关 键 词：玉米 大斑病 高压液相色谱 红外光谱 大斑病菌 特异性毒素组分 纯化 分离 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]