毛细管胶束电动色谱分离测定对硫磷、甲基对硫磷、水胺硫磷和克百威  

Separation and assay of parathion, methyl parathion, carbofuran and isocarbophos by micellar electrokinetic capillary electrophoresis

作　　者：李珊[1] 张兰[1] 陈国南[1] 

机构地区：[1]福州大学化学化工学院,福建福州350002

出　　处：《福州大学学报（自然科学版）》2003年第2期219-221,共3页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省自然科学基金资助项目 (D0 110 0 0 5 ) ;福建省科技厅科研基金资助项目 (2 0 0 0Z14 9)

摘　　要：用毛细管胶束电动色谱成功地分离 4种农药对硫磷、甲基对硫磷、水胺硫磷和克百威 .研究了电泳缓冲液的pH、浓度、及表面活性剂浓度等影响因素 ,在选定的最佳分离条件pH7.4、2 0mmol L硼酸 -四硼酸钠缓冲液 +30mmol LSDS下 ,4组份在 9min内得到基线分离 .检测限分别为 :对硫磷 2 .0 μg mL、甲基对硫磷 1.8μg mL、水胺硫磷 0 .8μg mL及克百威 1.0 μg mL .该方法成功地应用于模拟土壤样品中农药残余物的测定 ,回收率为 97.0 % - 10 3.8% .A method for the simultaneous separation and determination of parathion, methyl parathion, carbofuran, isocarbophos by micellar electrokinetic capillary electrophoresis has been developed. The conditions for separation and detection of a mixture of four pesticides have been optimized, and the experiment results showed that the investigated compounds could be successfully separated within 9?min by using a borate/SDS buffer as the carrier, and 20kV as the applied voltage. The detection limits are in the arrange of 0.8?μg/mL -2.0?μg/mL. This method was successfully applied to the analysis of pesticides in imitative soil sample. The recoveries of four pesticides are in the range of 97.0%-103.8%.

关 键 词：毛细管胶束电动色谱 分离 对硫磷 甲基对硫磷 水胺硫磷 克百威 农药残留量 含量测定 有机磷农药 

分 类 号：X839.2[环境科学与工程—环境工程] O657.7[理学—分析化学]