石竹试管苗成花与多胺代谢的研究  

Relationship between polyamine metabolism and flowering of Dianthus chinensis L. in vitro

作　　者：桂仁意[1] 曹福亮[1] 沈惠娟[1] 谢寅峰[1] 

机构地区：[1]南京林业大学森林资源与环境学院

出　　处：《北京林业大学学报》2003年第2期32-35,共4页Journal of Beijing Forestry University

摘　　要：以无根石竹试管苗为试材 ,通过外源多胺及多胺生物合成抑制剂调控石竹试管成花 ,研究其内源多胺代谢与成花的关系 .研究表明 ,外源多胺及多胺生物合成抑制剂影响石竹内源腐胺 (Put)、亚精胺 (Spd)、精胺 (Spm)及二丙胺(Dap)的代谢水平 .5× 10 - 2 mmol L的外源Spd、Spm有利于石竹试管苗成花 ,可将成花率由 36 7%分别提高到 6 0 %和4 8 3% .5mmol L的二氟甲基鸟氨酸 (DFMO)、环己胺硫酸盐 (CHAS)完全抑制成花 ,相同浓度的二氟甲基精氨酸 (DF MA)对成花影响不大 ,说明鸟氨酸脱羧酶 (ODC)比精氨酸脱羧酶 (ADC)作用更大 。Dianthus chinensis L.plants without roots in vitro were cultured on MS media, supplemented with polyamines and polyamine biosynthetic inhibitors.The aim was to study the relationship between polyamine metabolism and flower formation. The results showed that polyamines and polyamine biosynthesis inhibitors influenced the metabolism of putresine (Put), spemidine(Spd),spemine(Spm) and diaminopropane (Dap).5×10 -2 mmol/L Spm and Spd favoured flower formation, and the ratio of flower formation could be raised to 60% and 48.3% from 36.7% respectively. 5 mmol/L difluoromethylornithine (DFMO) or cyclohexyl ammolonium sulphat (CHAS) prohibited flower formation entirely, while difluoromethylarginine (DFMA) with the same concentration didn't, which indicated that ornithine decarboxylase (ODC) played a more important role than arginine decarboxylase (ADC) and the metabolism of Spd had a close relationship with flower formation of D.chinensis L.

关 键 词：石竹 试管苗 多胺代谢 成花 多胺生物合成抑制剂 

分 类 号：S681.5[农业科学—观赏园艺]