mRNA差别显示技术及其在植物基因研究中的应用  

Technique of mRNA differential display reverse transcription PCR and its application in gene research of plants

作　　者：马月萍[1] 戴思兰[1] 

机构地区：[1]北京林业大学园林学院

出　　处：《北京林业大学学报》2003年第2期81-84,共4页Journal of Beijing Forestry University

基　　金：国家林业局"948"引进项目 (2 0 0 1 2 4)

摘　　要：mRNA差别显示技术是近年发展起来的一种用于分离和克隆正常与异常细胞之间差异表达基因的PCR方法 ,是目前筛选差异表达基因的有效方法之一 .自问世以来在动物遗传育种方面取得了很大的成绩 ,广泛用于人类及其他动物生长发育基因调控、基因克隆的研究 .在植物基因研究方面应用起步较晚 ,现已开始用于植物基因分析、杂种优势机理等方面的研究 .在此 ,该文介绍了mRNA差别显示技术的原理、步骤、优缺点及其发展 ,并对其在植物特异基因分离方面的研究及其应用前景做了探讨 .The mRNA differential display reverse transcription PCR(DDRT-PCR) is a technique of cloning genes which are expressed differently between the good cells and bad cells and is an effective method too.It has been widely used in genetic regulating and breeding of animals and gained important achievements. DDRT-PCR was used later in the research of plant genes, and it has been used to analyse gene and heterosis principle of plants now. The principles of DDRT-PCR technique was introduced in this paper and its procedure, strong and weak points were described too.Furthermore,this paper discussed the application of the technique on the research of plants.

关 键 词：MRNA差别显示技术 植物基因研究 应用 技术原理 特异基因分离 

分 类 号：Q943.2[生物学—植物学]