虎纹蛙病毒的ATP酶基因克隆和序列分析  

Cloning and Analysis of RTV ATPase Gene

作　　者：苗素英[1] 张旻[1] 叶巧真[1] 王晓红[1] 江静波[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2003年第2期74-77,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(990255)

摘　　要：利用对应于蛙病毒-3型(FV3)ATP酶基因(ATPase)的162-178 nt和1 186-1 174nt碱基序列作为引物,采用PCR方法扩增得到虎纹蛙病毒(RTV)的ATP酶基因,并对该基因进行克隆、测序和分析.基因读码框大小为945bp,预计可编码一相对分子质量为35 500的蛋白质.氨基酸序列比较结果,RTV的ATPase基因与虹彩病毒科蛙病毒属的代表种FV3的一致性最高,为87.9%,与该科其它脊椎动物病毒的一致性在50%～52%之间.ATP酶蛋白质结构域分析可知该基因编码的ATP酶含有Walker型ATP酶AAA族蛋白质的全部结构域,其中既具有Walker型ATP酶所具有的Walker A和Walker B保守区,还含有AAA族蛋白质基因所具有的SRH高度保守区,因此,该基因是一完整的活性蛋白编码基因.The putative adenosine triphosphatase (ATPase) gene of Rana tigrina ranavirus (RTV) was amplificated, cloned and sequenced. The ORF of ATPase gene consists of 945 bp, which codes for a protein of 315 aa with a predicted molecular mass of 35 500. The identity of the ATPase gene was 88% and 52% to that of frog virus 3 (FV3) and lymphocystis disease virus (LCDV-1), respectively. The RTV ATPase protein was belonged to AAA (for ATPase associated with diverse cellular activities) protein family of the Walker-type ATPases. There were all of three highly conserved motifs (Walker A, Walker B and SRH) of AAA protein within the amino acid sequence of the RTV ATPase.

关 键 词：虎纹蛙病毒 虹彩病毒科 ATP酶基因 基因克隆 序列分析 氨基酸序列 水产养殖 病原 

分 类 号：S947.23[农业科学—水产养殖] Q939.4[农业科学—水产科学]