Chromatographic Characterization of Molecularly Imprinted Microspheres Synthesized by Aqueous Microsuspension Polymerization: Influences of pH, Kinds and Concentration of Buffer on Capacity Factors  

作　　者：赖家平 卢春阳 何锡文 

出　　处：《Chinese Journal of Chemistry》2002年第10期1012-1018,共7页中国化学（英文版）

基　　金：ProjectsupportedbytheNationalNaturalScienceFoundationofChina (No .2 0 175 0 0 9)andtheNaturalScienceFoundationofTianjin(No.0 0 36 0 2 2 11)

摘　　要：Molecularly imprinted microspheres (MIMs) were prepared using 4-aminopyridine (4-AP) as template molecule by aqueous microsuspension polymerization. The MIMs were packed into stainless steel column (250×4.6 mm I.D.) for selective separation of 4-aminopyridine (4-AP) and 2-aminopyridine (2-AP). The influences of pH, kinds and concentration (c) of buffer on capacity factors were investigated in detail. The relationships of capacity factor (k′) with pH and concentration of buffer are quantitatively described firstly. The effects of pH of phosphate and acetate buffer on capacity factors are very different. The relationship between k′ and pH can be described by the following equation: k′=-8.23 + 9.23 pH-0.99 pH 2 (in phosphate buffer) with R 2=0.9775 and k′=6.79-3.76 pH + 0.68 pH 2 (in acetate buffer) with R 2=0.9866. Furthermore, the capacity factors were also greatly affected by the concentration of acetate buffer in mobile phase while non-imprinted molecule is poorly changed. It increases with decreasing the concentration of buffer-especially in low concentration buffer (c acetate<0.02 mol/L, final concentration in mobile phase). The fit curve of log k′ to log c is described by equation: log k′=-0.571-1.256×log c-0.186×(log c) 2 with R 2=0.9979. The ratio of acetate buffer to methanol was investigated and the optimal ratio for separation of 4-AP and 2-AP is below 1∶7.5 (V/V).Molecularly imprinted microspheres (MIMs) were prepared using 4-aminopyridine (4-AP) as template molecule by aqueous microsuspension polymerization. The MIMs were packed into stainless steel column (250×4.6 mm I.D.) for selective separation of 4-aminopyridine (4-AP) and 2-aminopyridine (2-AP). The influences of pH, kinds and concentration (c) of buffer on capacity factors were investigated in detail. The relationships of capacity factor (k′) with pH and concentration of buffer are quantitatively described firstly. The effects of pH of phosphate and acetate buffer on capacity factors are very different. The relationship between k′ and pH can be described by the following equation: k′=-8.23 + 9.23 pH-0.99 pH 2 (in phosphate buffer) with R 2=0.9775 and k′=6.79-3.76 pH + 0.68 pH 2 (in acetate buffer) with R 2=0.9866. Furthermore, the capacity factors were also greatly affected by the concentration of acetate buffer in mobile phase while non-imprinted molecule is poorly changed. It increases with decreasing the concentration of buffer-especially in low concentration buffer (c acetate<0.02 mol/L, final concentration in mobile phase). The fit curve of log k′ to log c is described by equation: log k′=-0.571-1.256×log c-0.186×(log c) 2 with R 2=0.9979. The ratio of acetate buffer to methanol was investigated and the optimal ratio for separation of 4-AP and 2-AP is below 1∶7.5 (V/V).

关 键 词：molecularly imprinted microspheres aqueous microsuspension polymerization chromatographic stationary phase influence factors 

分 类 号：O658[理学—分析化学]