桐花树种群遗传变异与环境变量的关系  

Relationships Between Environmental Factors and Genetic Variation of Aegiceras corniculatum Populations

作　　者：黎中宝[1] 林鹏[1] 

机构地区：[1]厦门大学生命科学学院福建厦门

出　　处：《生态学报》2002年第11期1912-1916,共5页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目 (3 9670 1 3 5 )

摘　　要：研究了 9个桐花树 (Aegicerascorniculatum)种群遗传变异与环境变量的关系。结果表明环境因子与桐花树种群的Aat-2 A,Est-2 A,Est-2 B Mdh-1 A,Mdh-2 A Mdh-2 B Mdh-2 C Mdh-2 D等 8个等位基因相关性显著 ,尤其是前 4个等位基因 ,说明环境因子对这八个等位基因具有选择压力 ,他们的变化与环境变量有关。Genetic variation of 9 Aegiceras corniculation populations were investigated using the assay of vertical slab polyacrylamide gel electrophoresis. The concentration of concentrated gel and segregated gel were 2 5% and 7 0%, pH was 6 7 an 8 9, respectively. 13 Loci encoded by 5 enzyme systems were studied. A total of 5 enzymes namely were surveyed: aspartate aminotransferase (AAT, E.C.2.6.1.1 ), alcohol dehydrogenase (ADH, E.C.1.1.1.), esterase(EST, E.C.3.1.1 ), malic enzyme (M E,E.C.1.1.1.40), malate dehydrogenase (MDH,E.C.1.1.1.37). data analysis was submitted to the BIOSYS 1 for calculations of population genetic parameters. the allele frequencies ( A ) and observed heterozygosities ( Ho ) for each locus were determined by census of the populations data, the expected heterozygosities ( He ) within populations was estimated from average values among all samples in each species, A locus was considered to be polymorphic ( P 0.95% ) if the frequency of most common allele was equal or less than 0.95 at one or more localities. Environmental variables includes soil factors 、geographical factors and atmospheric factors in this study. There are organic matter 、total nitrogen、total phosphatidate、total salt and pH in soil, longitude and latitude in geographical factors, and the average temperature of a year and the fall of a year in atmospheric factors. Nine populations of Aegiceras corniculatum were selected to examine the relationships between environmental factors and genetic variation. The results indicates that the environmental factors have signeficantly relation with 8 alleles including Aat 2A, Est 2A, Est 2B Mdh 1A, Mdh 2A Mdh 2B Mdh 2C Mdh 2D, especially former 4 alleles. These results showed that the environmental factors which relate with the alleles have selective pressure on the 8 alleles, and the 8 alleles have some connection with the environmental factors.

关 键 词：桐花树 种群遗传变异 环境变量 关系 

分 类 号：S718.5[农业科学—林学]