重组汤对腮腺炎病毒作用的体外实验研究  

Experimental study on effect of recombined decoction on mumps virus

作　　者：张正云[1] 杨来智[2] 刘秦仲[1] 李国华[3] 陆学东[2] 

机构地区：[1]西安交通大学陕西医院口腔科,西安710068 [2]西安交通大学陕西医院病毒研究室,西安710068 [3]西安交通大学陕西医院药剂科,西安710068

出　　处：《中华实验和临床病毒学杂志》2002年第3期274-277,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　进一步求证重组汤对腮腺炎病毒体外灭活作用的特异性。方法　采用组织细胞培养技术 ,对腮腺炎病毒、单纯疱疹病毒 (Ⅰ、Ⅱ型 )、风疹病毒、巨细胞病毒、带状疱疹病毒、流感病毒、副流感病毒、腺病毒、呼吸道合胞病毒进行培养的过程中 ,给以实验药物攻击 ,观察病毒被灭活的过程。结果　实验药物在体外对腮腺炎病毒具有显著的抑制和杀伤作用 ,而对单纯疱疹病毒 (Ⅰ、Ⅱ型 )、风疹病毒、巨细胞病毒、带状疱疹病毒、流感病毒、副流感病毒、腺病毒、呼吸道合胞病毒无抑制作用。若增大实验药物剂量则对细胞本身的增殖生长有很大的副作用。结论　重组汤在体外实验中 ,对腮腺炎病毒具有抑制和杀伤作用的特异性。Objective To evaluate possible inactivating effect of recombined decoction in on mumps virus. Methods By adopting tissue cell culturing technology, a group of viruses including the mumps virus, herpes simplex virus(typeⅠ、Ⅱ), rubella virus, cytomegalovirus(CMV), herpes zoster virus, influenza virus, parainfluenza virus, adeno viruses, respiratory syneytial virus (RSV) were cultured.The cells infected with the viruses were treated with the decoction.Results The decoction showed remarkable inhibitory and killing effects on the mumps virus while had no obvious inhibitory and killing effects on host's cells,herpes simplex virus(typeⅠ,Ⅱ), rubella virus, cytomegalovirus(CMV), herpes zoster virus, influenza virus, parainfluenza virus, adeno viruses, respiratory syneytial virus(RSV). Conclusion The decoction had obvious inhibitory and killing effects on mumps virus during single layer cells culture.

关 键 词：腮腺炎病毒 重组汤 体外实验研究 抗病毒作用 中草药 

分 类 号：R285.5[医药卫生—中药学]