1990～2000年间我国乙型流感病毒HA1基因演变的特征  

Characterization of HA1 gene of influenza B virus circulated in 1990 through 2000 in China

作　　者：陈继明[1] 郭元吉[1] 郭俊峰[1] 董婕[1] 

机构地区：[1]中国预防医学科学院病毒学研究所流感室,北京100052

出　　处：《中华实验和临床病毒学杂志》2002年第3期278-280,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解 1990～ 2 0 0 0年间我国乙型流感病毒HA1基因的演变特征。方法　提取病毒RNA ,经逆转录和聚合酶链式反应扩增后测序 ,测定的序列和Genbank中已有的相关序列进行比较。结果　①我国乙型流感病毒在此期间一直存在两个差别较大的谱系 ,除 1994年和 1997年Victoria谱系为主外 ,其余各年均以Yamagata谱系为主 ;② 1992年以后Yamagata谱系又分化出两个组 ,彼此间氨基酸序列差异达 6 % ;③ 1990～ 2 0 0 0年间非回复突变的、大的变异株引导乙型流感的流行 ;④除了个别毒株之外 ,同一年份我国不同地区流行的属于同一谱系的乙型毒株HA1基因序列差别不大。结论　我国乙型流感病毒 1990～ 2 0 0 0年间一直存在两个差别较大的谱系 ,其中Yamagata谱系在此期间又分化出两个组 ,谱系的更换和同一谱系内出现大的变异株具有重要的流行病学意义。Objective To characterize HA1 gene of influenza B virus circulated in 1990 through 2000 in China. Methods Viral RNA was extracted and transcribed into cDNA by reverse transcriptase and amplified by PCR.The product of PCR was purified and sequenced by ABI377.The sequence data were analyzed with epidemic records. Results ①Two major lineages of influenza B virus always circulated during the period of 1990\|2000 in China; the Yamagata lineage was the main lineage,but in 1994 and 1997 the Victoria lineage was more active.②During 1992\|2000 the Yamagata lineage evolved into two minor groups whose distance in HAI amino acid sequences was about 6%.③Large and non\|reverse mutators led the development of influenza B epidemics in 1990\|2000 in China.④Except for a few strains,there was little difference among the influenza B viruses of the same major lineages circulated in the same year in China.Conclusion Two major lineages of influenza B virus always circulated during the period from 1990\|2000 in China,and the Yamagata lineage diverged into two minor groups in recent years.Exchanges of the lineages and the appearance of large non\|reverse mutators possibly had important epidemic significance.

关 键 词：乙型流感病毒 HA1基因 变异 1990~2000年 中国 

分 类 号：R373[医药卫生—病原生物学]