UTR PCR技术对婴儿中TT病毒感染情况的研究  

Application of UTR PCR in Scanning of TT Virus Infection in Infant

作　　者：曹志强[1] 彭宜红[2] 王清[1] 彭进[1] 胡列谱 

机构地区：[1]江西省九江市妇幼保健院儿内科,江西九江332000 [2]北京大学医学部病原生物学系病理生理教研室,北京100083

出　　处：《江西医学院学报》2002年第6期138-140,142,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的对九江市健康婴儿血清中TTV感染情况进行检测 ,以期阐明TTV在婴儿中的感染状况及传播途径。方法用改良UTRPCR法 ,检测婴儿血清中TTVDNA。结果 :用改良UTRPCR检测 86例婴儿中 5 1例 (5 3.5 % )TTVDNA为阳性 ,其中 <30日龄、1～ 6月龄和 7～ 12月龄婴儿中 ,TTVDNA检出率分别为 0、33.3%、95 .0 %。结论九江市95 %的半岁以上婴儿感染有TT病毒 ,婴儿中TTV的原发感染可能是出生后从环境中获得 ,并且随其年龄增长感染逐渐增多。Objective: To shed light on the prevalence of TTV infection and the transmission route of TTV infection in infants.Methods:Modified UTR PCR was performed to test TTV DNA in serum samples from 86 infants.Results:TTV DNA was detected by UTR PCR in 51(53.5%)in all the infants, while among infants of 30 days,1～6 months and 7～12 months of age,TTV DNA was detected by UTR PCR at a frequency of 0,33.3%,and 95.0%, respectively.Conclusion:The prevalence of TTV DNA detected by UTR PCR was 95% in infants of 7～12 months after birth in Jiujiang city,age dependent increase of TTV infection was observed in early childhood. The environmental sources are considered to be the most common route of TTV acquisition as the primary infection in infants.

关 键 词：UTRPCR技术 婴儿 TT病毒感染 聚合酶链反应 分子流行病学 

分 类 号：R725.11[医药卫生—儿科]