S-腺苷-L-蛋氨酸的分离纯化及其稳定产品的制备  

Purification of S-adenosyl-L-methionine and preparation of its stable salt products

作　　者：刘惠[1] 吴坚平[1] 林建平[1] 岑沛霖[1] 

机构地区：[1]浙江大学(玉泉)生物工程研究所,浙江杭州310027

出　　处：《中国生化药物杂志》2002年第6期284-287,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的 从酵母萃取液中分离纯化S-腺苷-L-蛋氨酸(SAM)并制备其稳定的盐产品。方法 以苦味酸作为选择性沉淀剂,结合有机溶剂结晶方法沉淀SAM盐产品。结果 确定了影响各步得率的关键因素和最适条件。结论　硫酸盐的制备得率(从酵母到成品)可达60％以上,而硫酸、对甲苯磺酸双盐的得率可达50％以上。Purpose Separation and purification of S-adenosyl-L-methionine (SAM) from yeast cells and the preparation of its stable salt products were investigated. Methods Picric acid was used to precipitate SAM from the cell extracts selectively. Organic solvents were adopted to precipitate SAM's salt products. Results The key factors affecting each step and the optimized conditions were determined. Conclusion Total yields of 60% and 50% could be reached for the preparations of SAM's sulfate salt and double salt of sulfate and p-tosylate respectively.

关 键 词：S—腺苷—L—蛋氨酸 分离 纯化 盐产品 制备 

分 类 号：TQ464.7[化学工程—制药化工]