黄芩甙对H_2O_2诱导星形胶质细胞NF-κB/IκBα表达的影响  

Effect of Baicalin on the Expression of Nuclear Factor-κB/IκBα Induced by Hydrogen Peroxide in Astrocytes

作　　者：宋元宗[1] 杨于嘉[1] 贾延 

机构地区：[1]中南大学湘雅医院儿科,湖南长沙410008

出　　处：《中国当代儿科杂志》2002年第6期436-438,T001,共4页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金资助课题 (No .3 9870 2 5 1)

摘　　要：目的　研究黄芩甙和H2 O2 对星形胶质细胞中NF κB/IκBα表达的影响。方法　体外培养星形胶质细胞 ,分别应用H2 O2 、黄芩甙及两者联用处理 ,应用细胞免疫化学法检测NF κB分子P6 5亚基核移位情况 ,WesternBlot检测细胞中IκBα表达变化。结果　在星形胶质细胞中 ,H2 O2 可使P6 5由胞浆向胞核移位 ,并降低IκBα的表达水平 ;黄芩甙自身不会导致P6 5核移位及影响IκBα表达水平 ,但可减少H2 O2 所致P6 5亚基胞核移位及细胞内IκBα降低的程度。结论　黄芩甙能减轻H2 O2 所致星形胶质细胞IκBα异常降低及NF κB异常活化。Objective To investigate the effect of baicalin, a flavonoid extracted from the radix of Scutellaria baicalensis Georgi, on the expression of nuclear factor (NF) κB/IκBα induced by hydrogen peroxide (H 2O 2) in astrocytes. Methods Astrocytes were cultured in vitro, and treated with various concentrations of baicalin or/and H 2O 2. Immunocytochemistry was used to determine the translocation of P65 from cytoplasm to nucleus; and Western Blot was used to determine the expression change of IκBα in astrocytes. Results In astrocytes, H 2O 2 could induce the translocation of P65, a subunit of NF κB, from cytoplasm to nucleus, and reduce the level of IκBα expression, while baicalin could inhibit this effect. Conclusions Baicalin may decrease the degree of IκBα degradation and NF κB activation induced by H 2O 2 in astrocytes.

关 键 词：黄芩甙 H202 诱导 星形胶质细胞 NF-κB/IκBα 表达 过氧化氢 核因子 中药 脑水肿 保护作用 

分 类 号：R285.5[医药卫生—中药学]