新发现的毛冠鹿的一核型与性染色体探讨  

A New Karyotype of Elaphodus Cephalophus and Discussion of Its Sex Chromosomes

作　　者：孔亚慧[1] 张锡然[1] 曹祥荣[1] 徐志鹏[1] 胡均 徐春茂 

机构地区：[1]南京师范大学生命科学学院,南京210097 [2]安徽省皖南野生动物救护中心,休宁245400

出　　处：《南京师大学报（自然科学版）》2002年第4期77-80,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(39970388).

摘　　要：　以原代培养和传代培养的方法培养成年雄性毛冠鹿的皮肤细胞,对其核型进行分析,发现了一种新的核型,二倍体染色体数为2n=48,染色体正好配成24对,其中有一对为异型染色体.该对异型染色体的一条为较大的端着丝粒染色体,长度仅次于第3号染色体,C带与薄层扫描显示其异染色质丰富.另一条为较大的近端着丝粒染色体,长度介于第2号与第3号染色体之间,比其他已知核型中相应的染色体长,几乎全为异染色质.G带分析表明该对异型染色体的异染色质区域均显示一些数目不等的浅带.本文通过核型分析,对毛冠鹿的性染色体进行了初步的探讨.The cell strain from the skin cells of a male Elaphodus cephalophus was established by primary culture and subculture. Analysis of chromosome showed a new karyotype. There were 24 pairs of chromosomes in somatic cell,including one pair of heteromorphic chromosomes,one of the pair chromosomes was a large telocentric chromosome showing rich heterochromatin by Cband and thin layer scaning technology,it was shorter than the No.3 chromosome,the other was a large subtelocentric chromosome almost consisting of heterochromatin,which was longer than the one in the other karyotypes resported. Gband analysis showed there were some faintlystained bands in the hereochromatinic regions of the pair of chromosomes. We analysed the karyotype and had a discussion on the sex chromosomes of Elaphodus cephalophus.

关 键 词：性染色体 毛冠鹿 核型 带型 薄层扫描显示 原代细胞培养 C带 G带 

分 类 号：Q959.842[生物学—动物学] Q34