蒿甲醚诱导日本血吸虫雌虫总抗氧化能力下降(英)  

Reduction of Total Antioxidant Capacity in Artemether-treated Female Schistosoma japonicum

作　　者：翟自立[1] 梅静艳[1] 焦佩英[1] 肖树华[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第6期354-357,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：青蒿素科技基金资助项目

摘　　要：目的 观察蒿甲醚对日本血吸虫成虫总抗氧化能力的影响。 方法 体外将血吸虫在含蒿甲醚和氯化血红素的培养液内培养24 h后,或体内感染小鼠经蒿甲醚300mg/kg治疗6～24 h后,测定虫体的总抗氧化能力。 结果体外50μmol/L的蒿甲醚与氯化血红素伍用引起雌虫总抗氧化能力明显下降。体内蒿甲醚作用血吸虫6 h,即见雌虫的总抗氧化能力明显下降。体内、体外试验中,蒿甲醚对雄虫的总抗氧化能力均无影响。 结论 蒿甲醚诱导雌虫总抗氧化能力下降。Objective To study the effect of artemether (Art) on total antioxidant capacity (T-AOC) in adult Schisto-soma japonicum. Methods In vitro, the T-AOC was determined in five-week old worms incubated without or with Art and/ or hemin for 24 h, and the worms were continuously incubated for 96 h, then worm survival was assessed. In vivo, T-AOC was determined in worms freshly recovered from mice 6 - 24 h after treatment with Art 300 mg/kg. Results Throughout 96 h incubation no worms were killed by 50 μmol/L Art or 50 μmol/L hemin alone, but approximately 80% of them were killed by Art plus hemin. Addition of reduced glutathione and vitamin E could significantly block the tidal action of the combined treatment. No effect on T-AOC was seen in the worms exposed to Art or hemin alone for 24 h, but the combined treatment led to a pronounced T-AOC reduction in female worms in vitro. Such a drug effect on female worms was demonstrated in vivo. After female worms were exposed to Art for 6 - 24 h in vivo, their T-AOC was significantly reduced by 40% - 64%. However, no drug effect on male worms' T-AOC was observed in vivo and in vitro exposed to Art plus hemin. Conclusion Art-induced T-AOC reduction in female worms may sensitize them to lethal damages of endogenous and exogenous oxygen radicals.

关 键 词：蒿甲醚 日本血吸虫雌虫 诱导 总抗氧化能力下降 血红素 

分 类 号：R383.24[医药卫生—医学寄生虫学] R978[医药卫生—基础医学]