Langmuir Aggregation of Thionin in Nucleic Acids and Its Application  

作　　者：郜洪文 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China School of Chemistry and Chemical Engineering, Anhui University, Hefei, Anhui 230039, China

出　　处：《Chinese Journal of Chemistry》2003年第2期170-174,共5页中国化学（英文版）

基　　金：ProjectsupportedbytheNaturalScienceFoundationofAnhuiProvince (No .0 10 45 3 0 1)andtheAnhuiProvincialScienceandTechnologyFoun dationforExcellentYouths.

摘　　要：The microsurface adsorption-spectral correction (MSASC) technique has been described. The formation of the double electrostatic films in nucleic acids causes the monolayer aggregation of stain molecules. The interaction of thionin (TN) with nucleic acids, ctD-NA, sDNA and yRNA, has been investigated at pH 7.8. Results show that the binding ratios of TN to ctDNA-P, sDNA-P and yR-NA-P are 1.27, 1.30 and 1.40, respectively, the binding constants of the aggregates are 2.8 · 104, 9.10 · 104 and 6.49 · 103 and their molar absorptivities are 1.04 · 104, 1.25 · 104 and · 104 L· mol?1, cm?1 at 630 nm. The aggregation may be applied to the spectrophotometric detection of nucleic acids samples.The microsurface adsorption-spectral correction (MSASC) technique has been described. The formation of the double electrostatic films in nucleic acids causes the monolayer aggregation of stain molecules. The interaction of thionin (TN) with nucleic acids, ctD-NA, sDNA and yRNA, has been investigated at pH 7.8. Results show that the binding ratios of TN to ctDNA-P, sDNA-P and yR-NA-P are 1.27, 1.30 and 1.40, respectively, the binding constants of the aggregates are 2.8 · 104, 9.10 · 104 and 6.49 · 103 and their molar absorptivities are 1.04 · 104, 1.25 · 104 and · 104 L· mol?1, cm?1 at 630 nm. The aggregation may be applied to the spectrophotometric detection of nucleic acids samples.

关 键 词：MSASC technique nucleic acid THIONIN double electrostatic film Langmuir aggregation 

分 类 号：O631.5[理学—高分子化学]