放射配体受体分析法对日本血吸虫体内5-HT受体的测定  

The Detection of 5-HT Receptor by RLBA in Schistosoma japonicum

作　　者：王丽平[1] 沈丽琳[1] 陈伟华[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《动物医学进展》2003年第1期94-96,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目(A9525)

摘　　要：采用放射配体-受体分析法(RLBA)测定了日本血吸虫体内5-羟色胺(5-HT)受体及螺环哌啶酮(spiroperidol)对5-HT受体与3H-5-HT结合反应的影响。结果证实:日本血吸虫体内存在5-HT受体,与配体5-HT可进行饱和的、特异的结合,其Scatchard图呈下凹曲线,表明5-HT受体的复杂性和不均一性。其最大结合容量(Bmax)为210.4fmol/mgprotein,解离常数(Kd)为0.585pmol/L,螺环哌啶酮对3H-5-HT与受体的结合无影响,计算Bmax为270.3fmol/mgprotein,Kd为0.327pmol/L,与前值比较,差异不显著(P>0.05)。5HT receptor in Schistosoma japonicum in vivo was determined by the radioligand binding assay(RLBA).The result showed that there were 5HT receptors in Schistosoma japonicum and the maximal association constant(Bmax)and Kd of 5HT receptor on Schistosoma japonicum were 210.4 fmol/mg protein and 0.585 pmol/L, respectively. The curve of Scatchard plot was of pit. It indicated that there was a greater association content and a higher affinity between the ligand and its 5HT receptor, but the binding was inhomogeneous and complicated.In addition, the result showed that spiroperidol had no effect on the binding between 5HT and it's receptor. The Bmax and Kd values were 270.3 fmol/mg protein and 0.327 pmol/L, respectively. The values were not significant compared with the above (P>0.05). The result suggested that the site binding with spiroperidol was not the same with 5HT. 

关 键 词：日本血吸虫 5-羟色胺 放射配体受体分析法 5-HT受体 螺环哌啶酮 

分 类 号：S852.735[农业科学—基础兽医学]