单诺沙星在雏鸡体内的药动学及其抗菌后效应研究  

Studies on Pharmacokinetics of Danofloxacin in Chickens and its Postantibiotic Effects

作　　者：刘芳萍[1] 佟恒敏[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《动物医学进展》2003年第1期108-110,共3页Progress In Veterinary Medicine

摘　　要：本论文以高效液相色谱(HPLC)内标法为定量手段,研究了单诺沙星经静注、口服两种途径给药后在雏鸡体内的药物代谢动力学特征;以菌落计数法测定了单诺沙星对大肠杆菌、金黄色葡萄球菌(金葡球菌)的体外抗菌后效应。静注和内服给药后血药浓度-时间数据分别符合无吸收因素二室开放式模型和一级吸收一室开放式模型。静注给药的主要药动学参数为:t1/2α0.3313h、t1/2β5.9940h、Vd7.5246L/kg、AUC5.6916μg/(mL·h)、CLB0.8935/(kg·h)。内服给药后主要药动学参数为:t1/2Ka0.3029h、t1/2K6.5128h、tmax1.2100h、Cmax0.5159μg/mL、AUC5.1329μg/(mL·h),生物利用度为90.18%。抗菌后效应(PAE)结果如下,浓度分别为0.5MIC、2MIC、4.MIC的单诺沙星对大肠杆菌的PAE测定值分别为(0.6464±0.0294)h,(1.2077±0.0284)h,(1.6529±0.0496)h,对金葡球菌的PAE测定值分别为(0.5660±0.0075)h,(1.1746±0.0057)h,(1.4913±0.0257)h。The pharmacokinetic characteristics of danofloxacin following intravenous and oral administration were conducted with chicken by using the (High performance liquid chromatography,HPLC) interal method in this study. The postantibiotic effects (PAE) of danofloxacin in vitro against E.coli and S.aureus were tested by routine colonial counting method.The pharmacokinetic characteristics in the chickens following single intravenous and oral administration were best described by twocompartment open model and onecompartment openmodel with firstorder absorption, respectively. The main pharmacokinetic parameters intravenously were as follows: t1/2α0.3313 h,t1/2β5.9940 h,Vd 7.5246 l/kg,AUC 5.6916 μg/(mL·h),CLB0.8935 l/(kg·h). The primary pharmacokinetic parameters of oral administration were showen below: t1/2Ka 0.3029 h,t1/2K6.5128 h,tmax 1.2100 h,Cmax 0.5159 μg/mL,AUC 5.1329 μg/(mL·h).The bioavailability was 90.18%. The postantibiotic effects of danofloxacin against E.coli and S.aureus in vitro at three different concentration levels were tested by routine colonial counting method. The PAE of danofloxacin against E.coli and S.aureus in vitro at the concentration levels of 0.5 MIC, 2 MIC and 4 MIC were (0.6464±0.0294) h,(1.2077±0.0284) h,(1.6529±0.0496) h for E.coli,and (0.5660±0.0075) h,(1.1746±0.0057) h,1.4913 h for S.aureus respectively.

关 键 词：单诺沙星 抗菌后效应 药物代谢动力学 雏鸡 

分 类 号：S859.796[农业科学—临床兽医学]