睾丸局部感染时Sertoli细胞IL-1、IL-6、TGF-β和FasL的变化  

Changes of IL-1, TL-6, TGF-p and FasL expressed by Sertoli cell in testis infection

作　　者：陈广洁[1] 李伟毅[1] 席晔斌[1] 王保国[1] 包士三[2] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025 [2]悉尼大学病理系

出　　处：《中国免疫学杂志》2003年第2期116-119,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(39970283)

摘　　要：目的:目的:研究大鼠Sertoli细胞在抗感染中的免疫调节作用。方法:以溶脲脲原体(UU)和致病性大肠杆菌(E.coli)直接注入大鼠膀胱模拟上行性感染的途径,分别在1周、2周、3周处死大鼠,分离取得睾丸组织作组织切片观察病理变化及从大鼠睾丸组织中分离获得高纯度的Sertoli细胞,用免疫组化方法比较正常组与UU感染组、致病性大肠杆菌组之间IL-1、IL-6、TGF-β和FasL表达的差异。结果:与正常组相比,UU和E.coli感染后,其IL-1分泌升高,IL-6分泌下降,TGF-β分泌升高,FasL表达也升高。结论:大鼠Sertoli细胞在抗感染免疫中,可通过IL-1、IL-6、TGF-β和FasL的表达来发挥免疫调节作用。Objective: To study the effect of immune regulation of the rat Sertoli cells in testis infection. Methods: UU and Escherichia coh'(E coli) were injected into the bladders of rats, which mimics the ascending infection pathway, while using culture medium injection as control. After 1 week, 2 weeks, 3 weeks respectively, the rats were sacrificed to observe the pathological changes in testis section. Sertoli cells were separated and obtained from rat testis, then experiments were designed to use the immunohistochemical staining to examine the different level of IL-1, IL-6, TGF-P and FasL among control ,UU and E coli infected groups.Results:Compared with control, in UU and E.coli infected group, the level of IL-1 increased ,the level of IL-6 decreased, the levels of TGF-P and FasL increased. Conclusion: Sertoli cell can play the role of immune regulation, with the expressing changes of IL-1, IL-6, TGF-P and FasL in the infection of testis in rat.

关 键 词：辜丸感染 SERTOLI细胞 免疫调节 抗感染作用 IL-1 IL-6 TGF-Β FASL 基因表达 动物实验 

分 类 号：R697.22[医药卫生—泌尿科学]