辛伐他汀对肺成纤维细胞凋亡的影响  

Effect of simvastatin on apoptosis in lung fibroblasts in vitro

作　　者：吴祖群[1] 李海浪[1] 杨莉[1] 沙莉[1] 邓红[2] 

机构地区：[1]东南大学附属中大医院儿科 [2]东南大学基础医学院病理科

出　　处：《江苏医药》2003年第2期94-96,共3页Jiangsu Medical Journal

摘　　要：目的　研究羟甲基戊二酸单酰辅酶 A(HMG CoA)还原酶抑制剂辛伐他汀对肺成纤维细胞凋亡的影响。方法　以胎儿肺组织为材料 ,胰蛋白酶消化法培养肺成纤维细胞 ,免疫组化方法鉴定 ,以辛伐他汀 5μM、1 0 μM、2 0 μM及不含辛伐他汀的溶剂作用 72小时 ,另以辛伐他汀 1 0 μM作用2 4、48、72、96小时 ,TUNEL及流式细胞仪定性及定量检测细胞凋亡。结果　 (1 )成功培养出肺成纤维细胞 ,光镜下细胞成梭形 ,免疫组化显示细胞表达纤维粘连蛋白和波动蛋白 ,不表达角化蛋白和结合蛋白。 (2 )TUNEL检测结果证实辛伐他汀可诱导细胞凋亡 ,光镜下也显示凋亡细胞的形态改变。流式细胞仪检测结果显示细胞凋亡率随辛伐他汀浓度及作用时间的增加而增加。Objective To investigate the effect of simvastatin on apoptosis in human lung fibroblasts in vitro.Methods Fibroblasts were prepared from human fetal lung tissue,cells were purified and identified immunohistochemically.The effect of simvastatin on apoptosis in fibroblasts was assessed by adding 5μM,10μM,20μM of the drug to culture flasks for 72 hours,and solvent alone withont any drug was added in the control group.The same drug concentration (10μM) for 24h,48h,72h,96h was also performed to determine apoptosis in different time space.TUNEL and flow cytometry were performed to determine cell apoptosis qualitatively and quantitively.Results 1.Lung fibroblasts were successfully cultured as evidenced by spindle shaped cells found under light microscope,and immunohistochemical stain showed that these cells were positive for vimentin and fibronectin but negative for keratin and desmin. 2.Result of TUNEL confirmed that simvastatin can induce fibroblast apoptosis and apoptotic changes occur under light microscope.Results of flow cytometry showed that the cell apoptosis increased parallel to time and drug concentration.Conclusion Simvstatin can induce apoptosis of lung fibroblasts in dose and time dependent manners.

关 键 词：辛伐他汀 肺成纤维细胞 影响 细胞凋亡 流式细胞仪 定性检测 定量检测 

分 类 号：R972.6[医药卫生—药品]