Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells  

作　　者：ZHUXiaodong YAOFeng TIENPo 

机构地区：[1]InstituteofMicrobiology,ChineseAcademyofSciences,Beijing100080,China [2]LaboratoryofTissueRepairandGeneTransfer,DivisionofPlasticSurgery,BrighamandWomen’sHospital,HarvardMedicalSchool,Boston,MA02115,USA

出　　处：《Progress in Natural Science:Materials International》2003年第2期114-118,T001,共6页自然科学进展·国际材料（英文版）

基　　金：Supported by the Major State Basic Research Development Program of China (G1999075602)

摘　　要：A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore, the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested.A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore, the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested.

关 键 词：tetracycline inducible expression system enhanced green fluorescent protein hepatitis B virus transdominant nega- tive proteins. 

分 类 号：R512.6[医药卫生—内科学]