HPLC测定人血浆中的酮洛芬  

Determination of Ketoprofen in human plasma by HPLC

作　　者：林舒[1] 蒋学华[1] 周静[1] 杨俊毅[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2003年第1期50-52,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的　建立人血浆中酮洛芬的HPLC测定法。方法　内标为布洛芬 ,用甲醇直接沉淀人血浆中蛋白质 ,上清液在HypersilBDSC18色谱柱上 ,以甲醇 -水 (6 0∶4 0 ,冰醋酸调pH 4 .0 )为流动相进行分离 ,流速 1ml·min-1,在UV2 5 7nm处检测。结果　酮洛芬和内标分离完全且无其他干扰 ,保留时间分别为 7.4 13、9.4 0 7min。线性范围为 0 .15～ 2 0 μg·ml-1,r =0 .9999,日内RSD为 1.0 8%～ 6 .0 1% ,日间RSD 1.11%～ 3.0 3% ,回收率为 97.4 %～ 10 5 .0 % ,血浆中酮洛芬的最低检测浓度为 0 .0 5 μg·ml-1。结论　所用方法简便、准确、重复性好 ,可用于口服酮洛芬缓释片后的血药浓度检测。OBJECTIVE To establish an HPLC determination method for Ketoprofen in human plasma.METHODS Using methanol to deposit protein directly.The Ketoprofen and the internal standard(Ibuprofen) were separated on C 18 column with a mobile phase consisting of methanol and water(60∶40,pH 4.0,by acetic acid), with flow rate of 1 ml·min -1 ,and the detection wavelength was 257 nm. RESULTS Ketoprofen was completely separated from the internal standard,and the retention time was 7.413 and 9.407 min,respectively. The calibration curve was linear within 0.15- 20 μg·ml -1 ,r= 0.9999. The recoveries of methodology were 97.4%-105.0%. Winthin day and between days, RSD were 1.08%-6.01% and 1.11%-3.03%,respectively.And the detection limit of Ketoprofen in human plasma was 0.05 μg·ml -1 .CONCLUSION This method is simple,accurate and reproducible. It has been successful in the determination of Ketoprofen in human plasma for pharmacokinetic studies.

关 键 词：酮洛芬 非甾体抗炎药物 血药浓度 高效液相色谱法 HPLC 药物动力学 

分 类 号：R971.1[医药卫生—药品] R969.1[医药卫生—药学]