3种血培养方法临床应用的评价  

Clinical Application of Three Kinds of Blood Culture Methods:An Evaluation

作　　者：朱冰[1] 胡勇[1] 郭志兵[1] 熊杰[1] 陈威巍[1] 钱江龙[1] 

机构地区：[1]成都军区总医院,四川成都610083

出　　处：《中华医院感染学杂志》2003年第1期89-90,共2页Chinese Journal of Nosocomiology

摘　　要：目的　评价临床常用的 3种血液培养方法的应用价值。方法　 3种不同浓度的 4种细菌各 70株 ,接种到 3种不同的培养瓶中进行培养 ,对其阳性检出时间、阳性检出率及相关因素进行综合性评估。结果　 mini VITAL系统对所测试的 3种不同浓度的细菌 (苛养菌除外 )阳性检出时间 3 .5～ 2 8.6h;对浓度为 10 0 CFU/ ml的苛养菌A群链球菌 ,m ini VITAL 48h阳性检出率可达 10 0 % ,双相血培养瓶阳性检出率为 17.1% ,自制血培养瓶的阳性检出率为 0 %。结论　 mini VITAL 全自动荧光血培养仪可缩短培养时间 ,结果快速准确 ;OBJECTIVE To evaluate the clinical application of three kinds of blood culture methods. METHODS Seventy strains of 4 species bacteria inoculated in three kinds of blood culture vails in three different concentrations were compared. The detection time and positive rate and some relative factors were evaluated.RESULTS The mini VITAL system detected all bacteria but streptococcus group A in 3.5 28.6 h. For detecting streptococcus group A in 100 CFU/ml, the detection positive rates in 48h with mini VATAL standard vails, biphase blood culture vails and self made blood culture vails were 100%, 17.1% and 0%, respectively. CONCLUSIONS The mini VITAL fluorescent automated blood culture system can reduce detection time. It is fast and accurate. Biphase blood culture vails are superior to self made blood culture vails on positive rate obviously.

关 键 词：血培养方法 临床应用 评价 阳性检出时间 阳性检出率 相关因素 细菌 

分 类 号：R372[医药卫生—病原生物学] R446.5[医药卫生—基础医学]