血小板内皮细胞粘附因子基因多态性与冠心病的关系  

Association of platelet endothelial cell adhesion molecule-1gene polymorphism with coronary heart disease

作　　者：宋福春[1] 陈爱华[1] 唐晓明[1] 张文秀[1] 钱学贤[1] 李江琪[2] 陆青[1] 

机构地区：[1]第一军医大学珠江医院心内科,广东广州510282 [2]第一军医大学珠江医院血液科,广东广州510282

出　　处：《第一军医大学学报》2003年第2期156-158,共3页Journal of First Military Medical University

摘　　要：目的观察血小板内皮细胞粘附因子(PECAM-1)在Leu125Val、Ser563Asn位点的基因多态性与冠心病之间的关系。方法将经心电图、心肌酶谱和冠状动脉造影检查确诊的156例病人作为冠心病组,同期住院经冠状动脉造影检查无冠脉病变的75例患者作为对照组,应用聚合酶链反应和限制性内切酶方法检测PECAM-1基因的多态性。结果冠心病组和对照组PECAM-1基因Leu125Val、Ser563Asn位点的基因型和等位基因分布均有显著性差异(P<0.05)。结论PECAM-1基因的多态性与冠心病的发病有明显相关性,可能是冠心病的遗传危险因素。Objective To investigate the association of Leu125Val and Ser563Asn polymorphism of the gene encoding platelet endothelial cell adhesion molecule-1(PECAM-1) with coronary heart disease. Methods This study included 156 patients with the diagnoses of coronary heart disease (CHD) and coronary lesions derived from electrocardiography, myocardial enzyme analysis and coronary angiography as the CHD group, and another 75 in-patients admitted within the same period who showed no signs of CHD in the above examinations constituted the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to examine the missense polymorphism of PECAM-1gene in the position of Leu125Val and Ser563Asn. Results There were significant differences between CHD and control group in terms of the allele frequencies and genotype distributions of PECAM-1 gene, and the differences were especially conspicuous in the allele frequencies of 125Val and 563Asn (P<0.05) and genotype distributions of 125Val/Val and 563Asn/Asn. Conclusion PECAM-1 gene polymorphism 1 may be a genetic risk factor for coronary heart disease.

关 键 词：血小板 内皮细胞 粘附因子 基因多态性 冠心病 心电图 心肌酶 

分 类 号：R541.4[医药卫生—心血管疾病]