高功率微波辐照对心肌细胞和心脏间质细胞生存能力和细胞活力影响的研究  

Cell activity and viability of cardiac myocyte and non-myocyte irradiated by high power microwave

作　　者：邓桦[1] 王德文[1] 刘杰[1] 曹晓哲[2] 赵梅兰[1] 谷庆阳[1] 董波[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]兰州军区兰州总医院病理科,甘肃兰州730050

出　　处：《西北国防医学杂志》2003年第1期7-9,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：全军医药卫生指令性课题 ( 0 1L0 2 3)

摘　　要：目的 :研究HPM对心肌细胞和心脏间质细胞生存能力和细胞活力的影响。方法 :HPM辐照体外培养乳鼠心肌细胞和心脏间质细胞 ,用流式细胞仪检测细胞凋亡和坏死 ;用MTT实验测定细胞活力。结果 :HPM可以对心肌细胞和间质细胞的功能和形态产生明显影响 ,引起二者的凋亡和坏死。结论 :心脏可能是HPM损伤的易感器官之一 ,并提示在探讨HPM对心脏的损伤时 。Objective:To study the changes of cell activity and viability of cardiac myocyte and cardiac non-myocyte which irradiated by high power microwave (HPM).Methods:cardiac myocyte and cardiac non-myocyte were irradiated by HPM. Then cell apoptosis and necrosis were detected by flow cytometer; and cell activity was examined by MTT experiment.Results:HPM can influence the function and morphology of the two kinds of cells obviously, and induce their apoptosis and necrosis.Conclusion:Heart maybe is one of the most susceptible organs to microwave. Meanwhile, the experiment suggest that we should attach importance to cardiac non-myomyte irradiated by HPM.

关 键 词：高功率微波辐照 细胞活力 心肌细胞 心脏间质细胞 细胞生存能力 细胞凋亡 HPM损伤 

分 类 号：R594.8[医药卫生—内科学]