LDH检测BALB/c小鼠CTL活性的初步研究  

A study of the specific cytotoxic T cell of BALB/c mice detected by LDH

作　　者：马巧玉[1] 王宇明[1] 郝飞[1] 

机构地区：[1]第三军医大学附属西南医院全军感染病研究所,重庆400038

出　　处：《第三军医大学学报》2003年第4期298-300,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 39770 6 82 )

摘　　要：目的　建立一个简便易行的动物模型及细胞毒性T细胞 (CTL)检测体系 ,为HCV感染中CTL作用研究奠定理论基础。方法　用SP2 O作为靶细胞 ,LDH检测HCV核心区多肽免疫BALB c小鼠的CTL活性。结果　HCV核心区多肽免疫BALB c小鼠后 ,可用LDH检测其CTL活性 ,CTL活性可被CPA10 ( 5～ 2 3位氨基酸 )增强及被CPA9( 3 9～ 74位氨基酸 )抑制。结论　LDH可稳定检测BALBObjective To establish a simple animal model and the cytotoxic T cell(CTL) detecting system for the studies of the effects of CTL on HCV infection. Methods The CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV was detected with lactic dehydrogenase (LDH) by using SP2/O cells as the target cells. Results CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV could be detected with LDH. The activity could be enhanced by CPA10(5～23 aa) but inhibited by CPA9(39～74 aa). Conclusion CTL activity in BLAB/c mice can be detected stably by LDH.

关 键 词：LDH 检测 BALB/cc小鼠 CTL活性 丙型肝炎病毒 HCV 

分 类 号：R512.63[医药卫生—内科学]