人血浆中吡格列酮的固相萃取高效液相色谱法测定  

Determination of Pioglitazone in Human Plasma by Solid-phase Extraction and HPLC

作　　者：施孝金[1] 陈妙英[1] 钱南萍 张静华[1] 王宏图[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040 [2]江苏泰州市人民医院,江苏泰州225300

出　　处：《中国医药工业杂志》2003年第2期90-92,共3页Chinese Journal of Pharmaceuticals

摘　　要：以吡格列酮的同系物 PIOGA为内标 ,采用 C2 固相萃取紫外检测的 HPL C分析法测定人血浆中吡格列酮的浓度。 C1 8分析柱 (15 0× 4.6 mm,5 μm) ,流动相 :水 -乙腈 -冰醋酸 (5 40∶ 46 0∶ 1.2 ,用氨试液调至 p H6 .0 ) ,流速 :1.0 ml/ min,检测波长 2 6 9nm。取血样 0 .5 m l,加入内标经 C2 固相萃取后进样 40μl。最低定量限 (L OQ)为10 ng/ ml,线性范围为 10～ 16 0 0 ng/The method for the determination of pioglitazone in human plasma by solid-phase extraction (C 2) and HPLC was presented. The chemical analogue of pioglitazone——PIOGA was used as the internal standard. The C 18 column was used with mobile phase of water-acetonitrile-glacial acetic acid (540∶460∶1.2, pH6.0, adjusted with ammouium hydroxide). The flow rate was 1.0 ml/min, and the UV detection wavelength was 269 nm. The internal standard solution (25 μl) was added into 0.5 ml of plasma sample, the mixture solution loaded onto the solid phase extraction cartridge (C 2, 100 mg, 1 ml) under low vacuum. The lower limit of quantification for pioglitazone was 10 ng/ml. The linear range was 10~1600 ng/ml.

关 键 词：血浆 吡格列酮 固相萃取 高效液相色谱法 测定 降血糖药 药理作用 

分 类 号：R977.15[医药卫生—药品]