妊娠期铅暴露对新生仔鼠脑组织MDA、SOD的影响及金属硫蛋白的诱导  

Effect of lead exposure during pregnancy on MDA,SOD and MT in the brain of newborn mice

作　　者：张敬旭[1] 符绍莲[1] 江河[1] 阮明[2] 张宝旭[2] 

机构地区：[1]北京大学公共卫生学院妇幼卫生学系,北京100083 [2]北京大学公共卫生学院卫生毒理学系

出　　处：《中国工业医学杂志》2003年第1期15-16,19,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　探讨妊娠期铅暴露对新生仔鼠脑组织丙二醛 (MDA)、超氧化物歧化酶 (SOD)的影响及对金属硫蛋白 (MT)的诱导。方法　采用灌胃方式于C57小鼠孕第 0d至妊娠结束进行醋酸铅染毒 ,仔鼠出生后第 1d处死 ,取脑组织测定MDA含量及SOD活性 ,同时测定脑组织中MT的含量。结果　妊娠期铅暴露使新生仔鼠脑组织MDA及MT含量升高 ,SOD活性降低 ,均呈明显的剂量 效应关系 (P <0 0 1)。结论　铅的脑发育毒性与其引起脑组织脂质过氧化产物MDA增高及抗氧化酶SOD活性降低有关 ;金属铅可诱导发育中脑组织MT的产生 ,提示MT在体内铅的转运。Objective To investigate the effect of lead exposure during pregnancy on the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and metallothionein(MT)in the brain of newborn mice.Method The mice of C 57 from the day 0 to end of gestation were treated with lead acetate through gastric feeding.The newborn mice were killed at the first day after birth.The levels of MDA,the SOD activity and the content of MT in the newborn mice brain were determined.Result Lead exposure of pregnant mice might enhance the level of MDA and MT in the brain of newborn mice,but the SOD activity in the brain was decreased.The changes were also dose-dependent on lead(P<0.01).Conclusion The brain developmental toxicity of lead is related to the increasing of MDA level and the decreasing of SOD activity in brain.In addition,lead can also induce the production of MT in the developing brain,that suggests that MT may play a certain role in the transportation,metabolism and detoxification of lead.

关 键 词：铅暴露 妊娠 脑组织 丙二醛 超氧化物歧化酶 金属硫蛋白 剂量-效应关系 神经发育毒性机制 

分 类 号：R135.11[医药卫生—劳动卫生]