大鼠骨髓基质干细胞体外诱导分化为类许旺细胞  

Differentiation of rat bone marrow stromal cells into Schwann cells-like in vitro

作　　者：侯赛云[1] 朱家恺[1] 

机构地区：[1]中山大学附属第一医院骨科显微外科医学部,广州市510080

出　　处：《中华显微外科杂志》2003年第1期39-41,共3页Chinese Journal of Microsurgery

基　　金：国家自然科学基金 (39830 1 0 0;39870 80 5);广州市科技攻关重大项目 (2 0 0 2Z1 E0 0 31 )

摘　　要：目的　研究大鼠骨髓基质干细胞在体外分化为类许旺细胞的方法。　方法　采用 β 巯基乙醇、全反式视黄酸、Forskolin、碱性成纤维细胞生长因子等依次按顺序诱导。神经胶质纤维酸性蛋白、S 10 0免疫细胞化学染色鉴定、计数诱导前后不同时间的细胞阳性表达率。　结果　诱导后骨髓基质干细胞形态改变 ,胞体呈椭圆形 ,有 2～ 3个纤细的细胞突起 ,成纵形栅栏状排列 ,免疫细胞化学染色神经胶质纤维酸性蛋白表达率为 42 5 %～ 6 8 7%、S 10 0表达率为 39 6 %～ 6 0 2 %。　结论　采用 β 巯基乙醇、全反式视黄酸、Forskolin、碱性成纤维细胞生长因子、血小板源生长因子、HRG可以诱导大鼠骨髓基质干细胞在体外分化为类许旺细胞。Objective To study the protocol that rat bone marrow stromal cells (BMSCs) can be induced to differentiate into Schwann cell-like in vitro. Methods BMSCs were treated with beta-mercaptoethanol followed by retinoic acid and cultured in the presence of forskolin,basic-FGF,PDGF and heregulin. The positive percentages of GFAP,S-100 protein expression were measured by immunocytochemistry SABC staining. Results After the induction,BMSCs displayed morphologies of Schwann cell,such as ellipsoidal cell bodies and fences-like array,with two or three sligh cell processes. The positive percentages of GFAP protein expression was from 42.5% to 68.7%,S-100 was from 39.6% to 60.2%. Conclusions Rat BMSCs could be induced to Schwann cell-like by beta-mercaptoethanol,retinoic acid,forskolin,basic-FGF,PDGF and heregulin

关 键 词：外周神经缺损 类许旺细胞 骨髓基质干细胞 碱性成纤维细胞生长因子 β-巯基乙醇 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]