许旺细胞在支架材料中的三维生长与迁移的研究  

Experimental study of Schwann cell growth and migration on biodegradable steroframe

作　　者：王光林[1] 林卫[2] 杨志明[1] 毕建红[3] 李胜富[4] 张杰[5] 

机构地区：[1]四川大学华西医院骨科,成都市610041 [2]四川大学华西第二医院 [3]四川大学法医与基础医学院免疫教研室 [4]四川大学华西医院移植免疫室 [5]四川大学华西医院电镜室

出　　处：《中华显微外科杂志》2003年第1期42-44,共3页Chinese Journal of Microsurgery

基　　金：国家自然科学基金重点项目 (39830 1 0 0 );国家自然科学基金 (30 1 0 0 1 90 );四川省卫生厅基金 (0 0 0 0 2 8)

摘　　要：目的　研究许旺细胞在聚乳酸 (PLA)无纺纤维布及聚羟基乙酸 (PLGA)纤维丝上的粘附、三维生长及迁移情况。　方法　将许旺细胞 /ECM凝胶悬液与PLA无纺纤维布及经胶原、多聚赖氨酸和ECM预处理的PLGA纤维丝复合培养 ,相差和激光扫描共聚焦显微镜观察许旺细胞在纤维丝中的三维生长和迁移。　结果　许旺细胞 /ECM悬液 ,与PLA无纺纤维布复合 ,随着凝胶的形成 ,大部份许旺细胞滞留在纤维网孔中 ,多数细胞和纤维丝贴附 ,形成多层排列的类似B櫣ｎｇｎｅｒ带状的许旺细胞柱硗赴芴皆赑LGA纤维丝上生长并沿纤维丝移行 ,ECM凝胶能显著增加贴附和移行细胞数量。　结论　ECM凝胶能促进许旺细胞在支架中的黏附、生长与迁移 ,是构建组织工程化人工神经的良好整合材料。Objective To observe the behavior of Schwann cell migration and wrapping up of fibres during co-culture of Schwann cells with steroframe of PLA and PLGA fibres. Methods Schwann cells were mixed into 30% ECM gel and co-cultured with PLA non-spinning fibre steroframes and seeded on one ends of PLGA fibres which were treated with collagen,polylysine or ECM. The attachment,growth and migration of Schwann cells were observed under phase contrast microscope and laser scan confocus microscope. Results (1)When Schwann cells were co-cultured with PLA steroframes,most of the Schwann cells kept in the fibre holes and attached on PLA fibres to form Büngner band likd structures. (2)When co-cultured with PLGA fibers,Schwann cells could attach and migrate on PLGA fibres and the number of Schwann cells were obviously increased when PLGA fibres were treated by ECM. Conclusions (1)ECM gel colud promote Schwann cells attachment,growth and migration on PLA steroframes and PLGA fibers. (2)ECM gel is a good integrating substance for constructing tissue engineering bioartificial nerve.

关 键 词：许旺细胞 支架材料 三维生长 聚乳酸 无纺纤维布 聚羟基乙酸 纤维丝 

分 类 号：R318.08[医药卫生—生物医学工程]