大鼠原位肝移植模型的手术技巧及并发症的预防  

Surgical technique of the model of orthotopic liver transplantation and prevention of operational complication in rat

作　　者：马毅[1] 何晓顺[1] 陈规划[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州市510080

出　　处：《中华显微外科杂志》2003年第1期45-47,共3页Chinese Journal of Microsurgery

基　　金：卫生部临床学科重点项目 (970 4 0 2 30 );广东省科委攻关基金 (99M0 4 90 2G)

摘　　要：目的　探讨建立一个稳定的大鼠原位肝移植模型的手术技巧以及术中和术后并发症的预防。　方法　在Kamada用“二袖套法”吻合血管的基础上进行改良。供体改经腹主动脉进行肝脏冷灌注 ;肝上下腔静脉用缝合法吻合 ,门静脉和肝下下腔静脉用袖套法吻合 ,胆总管采用单管内支架胆管端端吻合法。　结果　共施行大鼠原位肝移植 36 0次 ,手术成功率为 91 3 %。非干预组中 ,同系移植大鼠 1周存活率为 86 5 % ,3个月存活率达 80 7%。　结论　熟练的显微外科技术、细致的手术操作是预防术中和术后并发症的先决条件。受体无肝期的长短是决定动物存活的关键。Objective To investigate the surgical technique of establishing a reliable rat model of orthotopic liver transplantation and prevention of operational complication. Methods The model was established with modified cuff technique.The donor was perfused through the abdominal aorta with 20ml cold perfusate.The anastomosis of the suprahepatic vena cave(VC)was sutured end-to-end with 8-0 nylon line,and the continuity of infrahepatic VC and portal vein (PV)were established by means of cuff method respectively.The bile duct anastomosis was performed by internal stent. Results Three hundred and sixty case of rat orthotopic liver transplantation were performed and the successful rate of the mode was 91.3%.In non-intervention group,the survival rat was satisfactory,with a 86.5%of 1 week survival and a 80.7% of long-term survival (longer than 3 months). Conclusions The sophisticated microsurgical technique and the delicate surgical manipulation is the prerequisite of preventing operational complication,and shortening the anhepatic phase of recipient as soon as possible is the key to animal survival.

关 键 词：并发症 原位肝移植模型 手术 术后 

分 类 号：R657.3[医药卫生—外科学]