滩羊生长激素基因PCR-RFLPs反应体系的优化  

OPTIMIZATION OF PCR-RFLPS REACTION SYSTEM FOR TAN SHEEP GH GENE ANALYSIS

作　　者：于洪川[1] 魏智清[1] 张振汉[1] 陈如熙[2] 杜立新[3] 龚伟宏[3] 

机构地区：[1]宁夏大学生命科学学院,银川,750021 [2]山东农业大学,山东泰安,271018 [3]宁夏农牧厅畜牧工作站,银川,750001

出　　处：《宁夏农学院学报》2003年第1期29-30,33,共3页Journal of Ningxia Agricultural College

摘　　要：以宁夏滩羊基因组DNA为模板 ,研究影响滩羊PCR -RFLPs扩增的因素 ,实验结果表明 :特异性引物的浓度、Mg2 + 浓度、模板DNA的完整性 ,浓度、纯度、TaqDNA聚合酶、变性温度、退火温度和循环次数等诸多因素对PCR -RFLPs扩增影响较大。为此我们对PCR -RFLPs的反应体系和反应程序进行了研究和浓度梯度对比试验 ,建立了滩羊GH基因PCRThe study on factors affecting amplification of PCR-RFLPs for Tan sheep of Ningxia carried out by using Tan sheep genetic DNA as a template The result showed that the concentrations of specific primer Mg ++ , Taq polymerase and the purity and completion of template DNA, denaturing temperature, annealing temperature, the number of cycles played an important role in PCR-RFLPs analysis We studied and tested the concentration step's contrast for PCR-RFLPs reaction system and reaction process A technical system for Tan Sheep GH gene PCR-RFLPs was estab-lished

关 键 词：滩羊 生长激素 PCR—RFLPs 反应体系 优化 反应程序 

分 类 号：S826.2[农业科学—畜牧学]