双氢青蒿素体外抗卡氏肺孢子虫电镜细胞酶化学研究  

Effect of dihydroarteannuin on pneumocystis carinii by electromicroscopic enzymic cytochemistry in vitro

作　　者：倪小毅[1] 陈雅棠[1] 王健[2] 徐晨[3] 王红[3] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016 [2]深圳市第二人民医院肺科,深圳518035 [3]重庆医科大学电镜室,重庆400016

出　　处：《第三军医大学学报》2003年第5期381-384,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 396 70 6 58)

摘　　要：目的　观察双氢青蒿素对卡氏肺孢子虫虫体的酸性磷酸酶 (AcPase)、葡萄糖 6 磷酸酶 (G 6 Pase)和腺苷三磷酸酶 (ATPase)等 3种代谢酶的影响。方法　将Pc接种于有支持细胞的 5 0ml培养瓶中 ,4h后 ,试验组每瓶加入DHA 5 0μmol·L- 1 ,同时作对照组。用药后 12h ,作电镜酶细胞化学 ,观察上述 3种酶的定位及用药后各种酶超微结构水平上的变化。结果　AcPase反应产物用药组与不用药组无明显变化。G 6 Pase、ATPase反应产物用药组明显减弱。酶的阴性对照组无酶产物产生。结论　双氢青蒿素对卡氏肺孢子虫细胞内的AcPase无影响。双氢青蒿素对PcG 6 Pase、ATPase的活性有明显的抑制 ,可能是影响Pc细胞的代谢功能 ,最终抑制其增殖的原因之一。Objective To observe the effect of dihydroarteannuin(DHA) on acid phosphatase (AcPase), adenosine triphosphatase (ATPase) and glucose 6 phosphatase (G 6 Pase) of pneumocystis carinii (Pc). Methods Pc was inoculated into HepG 2 cells in 50 ml bottle. After incubation for 4 h, DHA 50 μmol/L was added. Samples were harvested after 12 h, and immediately incubated for AcPase, G 6 Pase and ATPase. The localization and ultrastructural changes were observed electromicroscopically. Results No difference was found between DHA treated group and control group in AcPase. The reactive product of G 6 Pase and ATPase decreased markedly in DHA treated group. Conclusion DHA has no effect on AcPase of pneumocystis carinii , but the inhibitive effect of DHA on the activities of G 6 Pase and ATPase by the influence on the metabolism function of Pc may be one of the causes resulting in the inhibition of proliferation of Pc.

关 键 词：双氢青蒿素 卡氏肺孢子虫 酸性磷酸酶 葡萄糖-6磷酸酶 腺苷三磷酸酶 代谢酶 

分 类 号：R531.5[医药卫生—内科学]