利用不同支架材料构建人工真皮的研究  

STUDY ON THE ARTIFICIAL DERMAL SKIN BUILT BY DIFFERENT SCAFFOLDS

作　　者：刘杰[1] 崔雪梅[1] 张燕[1] 赵梅兰[1] 谷庆阳[1] 吴小红[1] 徐龙河[2] 杨颖颙[3] 于淑贤[3] 王德文[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]解放军总医院麻醉科 [3]中国皮革和制鞋工业研究院

出　　处：《中国修复重建外科杂志》2003年第2期104-107,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：军事医学科学院创新基金重点资助项目 (990 51 0 6)~~

摘　　要：目的　探讨脱细胞基质、胶原蛋白基质网架、胶原凝胶的不同支架材料上构建人工真皮的可行性。　方法　采用酶消化法分离幼儿包皮成纤维细胞 ,并进行传代培养 ;将第 3代成纤维细胞种植于三种不同支架上 ,14天后利用 HE染色、透射电镜和扫描电镜对构建的人工真皮结构进行鉴定。结果　 1酶消化法得到皮肤成纤维细胞 ,可在含有 10 %小牛血清的 DMEM中传代培养 ;2成纤维细胞在三种不同支架上形成单层细胞 ,并可向胶原膜和胶原凝胶内部生长 ;3成纤维细胞在胶原膜和脱细胞基质内增殖不如胶原凝胶内活跃 ,但人工真皮收缩不明显 ,而胶原凝胶构建的人工真皮收缩明显。　结论　利用脱细胞基质、胶原蛋白基质网架、胶原凝胶支架材料构建的人工真皮具有较好的组织结构 ,是一种较理想的皮肤替代物 ,可作为移植物进行深入研究。Objective To build artificial dermis by using the acellular dermis matrix(ADM), collagen membrane and collagen gel as scaffolds. Methods The fibroblasts were isolated by enzyme from infant skin and were cultivated in the DMEM medium. After 14 days when the fibroblasts were seeded into 3 different scaffolds, the autografts were detected by HE staining, transmission electron microscope and scanning electron microscope. Results ①The fibroblasts obtained from the fullskin by enzyme could be passaged in the Dulbecco's modified Eagle's medium high glucose with 10% calf bovine serum. ②A layer of fibroblasts were found on the surface of three different scaffolds, the fibroblasts could grow into the collagen membrane and the collagen gel, but could not be found in the inner of ADM. ③Artificial dermis contracted slightly by inoculating fabrication on collagen membrane and ADM, and the fibroblasts on them were not active in proliferating; but the artificial dermis built by the collagen gel contracted obviously. Conclusion The artificial dermis built by ADM, collagen membrane and collagen gel as scaffolds have a preferable structure for an ideal substitute of skin, and can be used as the graft in the next experiments.

关 键 词：人工真皮 支架材料 脱细胞基质 胶原蛋白基质网架 胶原凝胶 

分 类 号：R318.19[医药卫生—生物医学工程]