生物衍生材料构建组织工程肌腱体内植入的实验研究  

EXPERIMENTAL STUDY ON IN VIVO IMPLANTATION OF DERIVED TENDON MATERIAL COMBINED WITH ALLOGENOUS TENDON CELLS IN MACACA

作　　者：周悦婷[1] 项舟[1] 阳富春[1] 李秀群[1] 杨志明[1] 

机构地区：[1]四川大学华西医院卫生部移植工程与移植免疫重点实验室:修复重建外科研究室.组织工程实验室,成都610041

出　　处：《中国修复重建外科杂志》2003年第2期152-156,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点基础研究发展规划项目 (973) :--组织工程肌腱的基础研究及临床应用 (G1 9990 5430 8) ;国家高技术发展规划资助项目 (863) (2 0 0 1 AA2 1 60 1 1 ) ;国家自然科学基金资助项目 (39870 2 0 2 ;3980 0 1 4 8) ~~

摘　　要：目的　探讨用同种异体肌腱细胞与生物衍生肌腱材料体外联合培养后植入体内 ,构建组织工程肌腱的可行性。　方法　选用四川猕猴 15只 ,手术造成纤维鞘管内屈指深肌腱 2 .5 cm缺损后 ,分三组。A组 :生物衍生肌腱材料构建的组织工程肌腱移植组 ;B组 :单纯生物衍生肌腱材料移植组 ;C组 :自体肌腱移植组。术后 1、2、3、6和 12周分期观察植入物的大体形态、组织学和超微结构 ,Brd U标记表达。　结果　 A组植入体内后肌腱细胞能继续增殖 ,细胞形态随着时间增加而逐渐趋于正常 ,形成的肌腱呈白色且有光泽、致密 ,组织学可见胶原纤维排列较为规则 ,12周肌腱细胞仍成活并分泌胶原 ,Brd U表达为阳性 ;B组植入体内 3周后材料逐渐变细 ,12周后材料被逐渐降解吸收出现中断 ;C组植入体内 2周后桥接部有纤维连接 ,排列较为规则 ,肌腱愈合。A组分别于 3、6、12周进行扫描电镜观察可见肌腱细胞排列均匀 ,胶原纤维相互连接形成网状 ,主体趋势与肌腱走行方向一致 ;透射电镜下可见细胞核仁清晰 ,细胞器丰富。随时间的增加 A、C组与 B组的差异明显增大。　结论　同种异体肌腱细胞与生物衍生肌腱材料复合构建的组织工程肌腱 ,植入免疫功能正常的动物体内能够再生出肌腱样组织 ,植入的肌腱细胞具有生命力 。Objective To investigate the possibility of repairing defected tendon with a tissue engineering tendon, combined culture of allogenous tenocyte and derived tendon. Methods Macaca tenocytes labelled by BrdU were seeded on the derived tendon. The flexor digitorum profundus of five fingers of left hand in 15 Macaca mulatta were resected and made 2.5cm defects as experimental model. They were divided into three groups according to repair methods (Group A: Combined culture of derived tendon materials and allogenous tendon cells; Group B: Derived tendon materials; Group C: Autograft). In different stages, the labeled BrdU of tendon cells were detected by immunohistochemical methods, and the morphological changes and ultrastructure of tendon cells were observed. Results In Group A, after in vivo implantation, the tenocytes could proliferate and synthesize collagen; the new tissue was white and glossy and the collagen fibers fused to form dense tendon structure as several weeks passed. Twelve weeks after implantation, the tenocytes still survived and synthesized collagen, the results of labelled cells were positive by immunohistochemical methods. By scanning electron microscopic observation, the tenocytes arranged regularly and evenly among the derived tendon; the collagen fibers formed a network and its main direction was accord with that of the derived tendon. Normal nucleus, nucleolus, and cell organelles were seen under transmission electron microscope. Conclusion Combined culture of tenocytes with derived tendon is able to make tendon like tissue. The structure of tissue engineering tendon is similar to that of normal tendon.

关 键 词：生物衍生材料 组织工程 体内植入 肌腱缺损 

分 类 号：R318.08[医药卫生—生物医学工程]