脱细胞基质与血管内皮细胞体外相容性的研究  

EXPERIMENTAL STUDY OF COMPATIBILITY BETWEEN ACELLUARIZED ALLOGENIC MATRIX AND ENDOTHELIAL CELL IN VITRO

作　　者：郭铁芳[1] 杨大平[1] 韩雪峰[1] 杜金荣[1] 张颖[1] 

机构地区：[1]哈尔滨医科大学附属第二医院整形外科,哈尔滨150086

出　　处：《中国修复重建外科杂志》2003年第2期165-168,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目 (30 1 70 970 ) ;黑龙江省自然科学基金资助项目 (D0 0 2 1 )~~

摘　　要：目的　将猪血管内皮细胞和异体猪血管脱细胞基质相结合 ,探讨开发一种制备血管移植物的新方法。方法　分别利用 0 .1%胰蛋白酶、0 .0 2 %乙二胺四乙酸钠 (EDTA)和 1.0 % Triton X- 10 0对猪主动脉作用 2 4小时和 176小时进行脱细胞。异体猪血管内皮细胞分离出来并进行体外细胞扩增 ,再将脱细胞的血管内表面种植上血管内皮细胞。通过光镜和扫描电镜检测脱细胞血管基质是否存在细胞成分和内皮细胞是否生长其上。　结果　酶 -化学除垢剂的脱细胞方法几乎使细胞完全脱落 ,且基质纤维的三维结构变得疏松。体外扩增的异体猪血管内皮细胞可以种植在脱细胞基质上并且具有伸展和增殖功能。　结论　 Triton X- 10 0和胰蛋白酶制备的脱细胞基质与异体猪血管内皮细胞具有良好的生物相容性 ,能结合到一起并在体外生成血管移植物。Objective To develop a new method for a tissue engineered vascular graft by combining endothelial cells and an acelluarized allogenic matrix. Methods Acellularized matrix tubes were obtained by a 0.1% trypsin and 0 02% EDTA solution for 24 hours and 1% Triton X 100 for 176 hours, respectively. Endothelial cells were isolated from alloaorta and expanded in vitro. Finally, the inner surface of acellularized matrix was reseeded with endothelial cells. Acellularity and reseeding were analysed by light microscopy and scanning electron microscopy. Results The acellularization procedure resulted in an almost complete removal of the original cells and the loose three dimensional(3D) matrix. The acellular matrix could be reseeded with expanded endothelial cells in vitro, and endothelial cells had the potential of spread and proliferation. Conclusion Acellular matrix produces by Triton X 100 and trypsin possesses satisfactory biocompatibility for allogenic endothelial cell. Vascular grafts can be generated in vitro by a combination of endothelial cells and allogenic acelluarized matrix.

关 键 词：血管疾病 血管移植 脱细胞基质 血管内皮细胞 体外相容性 组织工程 血管移植物 

分 类 号：R318.11[医药卫生—生物医学工程]