两种染色方法对聚丙烯酰胺凝胶电泳回收蛋白的影响  

Effect of two Staining Methods in SDS-PAGE on Protein Recovery

作　　者：周艳芬[1] 赵晓瑜[1] 李亚东[1] 乔环宇[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2003年第1期59-61,共3页Journal of Hebei University(Natural Science Edition)

摘　　要：以牛血清白蛋白(BSA)为材料进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE),分别采用考马斯亮蓝(CBB)G-250染色和咪唑-Zn反染,用电洗脱仪回收。它们的灵敏度分别为500ng和5ng;蛋白质回收率分别为37.9％和74.4％。BSA was run on SDS- PAGE, stained with Coomassie Brilliant Blue(CBB) G- 250, or reverse stained with Imidazole- Zinc, then recovered by using electroeluter. The sensitivities were 500 ng and 5 ng respectively for CBB G - 250 and Imidazole - Zinc; and the recoveries were 37.9% and 74.4% .

关 键 词：牛血清白蛋白 十二烷基硫酸钠—聚丙烯酰胺凝胶电泳 染色方法 蛋白质回收 马斯亮蓝 咪唑—Zn 

分 类 号：Q512.103[生物学—生物化学]