大鼠胰腺蛋白质组研究中双向电泳技术的初步建立  

Preliminary Establishment of Two-dimensional Gel Electrophoresis in Research of Pancreas Proteome of Rats

作　　者：程志祥[1] 熊俊[1] 倪雪峰[1] 顾冬民[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学及分子生物学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2003年第2期106-108,115,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学创新基金资助项目(Cx20005)

摘　　要：目的:初步建立大鼠胰腺蛋白质组研究中双向电泳技术,提高其分辨率及重复性。方法:应用大鼠成年和胚胎18天胰腺组织提取的总蛋白,对以载体两性电解质pH梯度为第一向的双向电泳关键因素与环节,如样品处理、凝胶制备、电泳参数等进行一系列的优化。结果:获得了分辨率及重复性均很好的双向电泳图谱。结论:通过对各种条件的优化,初步建立了双向电泳技术,为研究胚胎胰腺不同时期特异性蛋白的表达及分子标志打下基础。Objective: To establish the two-dimensional gel electrophoresis (2-DE) in research of pancreas proteome, and promote the resolution and reproducibility. Methods: A series of important factors, such as sample handling, gel preparation, electrophoresis parameters and so on were optimized in extraction of the proteins of pancreas from adult rats and 18-day embryo of rats. Results: The 2-DE patterns with good feature have been obtained. Conclusion: With optimization of each condition, two-dimensional gel electrophoresis (2-DE) has been preliminarily established.

关 键 词：双向电泳 胰腺 蛋白质组 胚胎 

分 类 号：R322.491[医药卫生—人体解剖和组织胚胎学] Q51[医药卫生—基础医学]