游泳对慢性低氧高二氧化碳大鼠一氧化氮代谢的影响  

Effect of swimming exercises on nitric oxide content and nitric oxide synthase activity in rats chronically exposed to hypoxia and hypercapnia

作　　者：龚永生[1] 郑绿珍[1] 胡良冈[1] 梅惠华[1] 李继武[1] 范小芳[1] 

机构地区：[1]温州医学院肺心病研究室,浙江温州325027

出　　处：《温州医学院学报》2003年第1期15-17,共3页Journal of Wenzhou Medical College

基　　金：浙江省卫生厅资助项目 (SY960 0 0 5 );温州市科委资助项目(S2 0 0 1A2 4)

摘　　要：目的 :探讨游泳锻炼对慢性低氧高二氧化碳大鼠一氧化氮 (NO)代谢及肺动脉平均压 (mPAP)的影响。方法 :SD大鼠随机分成舱外正常对照组 (NC)与慢性低氧高二氧化碳模型组。 4w后 ,模型组又随机分为模型对照组 (HH)与游泳锻炼组 (SE)。用比色法测定NO代谢产物 (NO2-/NO3-)的含量 ,用液体闪烁仪测定 [3H] 瓜氨酸的生成量 ,计算tNOS、iNOS和cNOS的活性。结果 :SE组的血浆、肺与右心室组织的NO2-/NO3-的含量和cNOS的活性明显高于HH组 ;同时 ,SE组的mPAP显著低于HH组。结论Objective:To investigate the effects of swimming exercises on nitric oxide(NO) and mean pulmonary artery psessuse(mPAP) in rats chronically exposed to hypoxia hypercapnia.Methods:Thirty one Sprague Dawley rats were randomly divided into two groups,normal control group and chronic hypoxia hypercapnia one. Rats were exposed to hypoxia hypercapnia for four weeks ,the latter group was subdivided into two:hypoxia hypercapnia control(HH) group and swimming exercises(SE) group. Contents of nitrate/nitrite,a stable metabolic product of NO in plasma,pulmonary and right ventricular homogenates were measured using colorimetric analysis. L citrulline was determined by liquid scintillometer,and the activities of NO synthase(iNOS,tNOS,cNOS) were counted.Results:The NO 2 -/NO 3 - contents and cNOS activities in plasma,pulmonary and right ventricular homogenate of SE group were markedly higher than those of HH group. Moreover,mPAP of SE group was obviously lower than that of HH group.Conclusion:Swimming exercises could increase NO production and lower mPAP of the rats chroniclly exposed to hypoxia hypercapnia.

关 键 词：一氧化氮 低氧 慢性 高血压 肺性 游泳 

分 类 号：R541.5[医药卫生—心血管疾病] R364.4[医药卫生—内科学]