参黄草合剂对大鼠急性肾损伤保护作用的实验研究  

The Experimental Study on the Protective Effects of the Shenhuangcao-mixture on Acute Renal Injury in Rats

作　　者：张国欣[1] 王秀芬[1] 张萍[1] 吴云霞[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院,哈尔滨150001

出　　处：《中国中医药信息杂志》2003年第2期21-22,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的:研究参黄草合剂对庆大霉素所致的急性肾损伤大鼠肾功能的保护作用。方法:取大鼠40只,其中10只作为正常对照组,其余30只制成急性肾损伤模型,并随机分为3组。参黄草组用参黄草溶液灌胃;包醛组用包醛氧淀粉灌胃;病模组(同正常组)用0.85%氯化钠液灌胃。治疗10d,观察各组血肌酐(SCr)、尿素氮(BUN)和尿酶(NAG)的变化;将大鼠处死后观察其肾组织的病理改变。结果:参黄草组治疗后SCr、BUN和NAG较病模组明显下降,并优于包醛组(P<0.01);光镜检查参黄草合剂可使肾小管上皮细胞肿胀、坏死等组织病理改变明显减轻,电镜下可见线粒体损伤等变化亦显著减轻。结论:参黄草合剂可改善急性肾损伤大鼠的肾功能,减轻水肿,阻止庆大霉素对肾小管上皮细胞的进一步损害。Objective:To study the protective effects of Shenhangcao-mixture on acute renal injury caused by gentamicin in rats. Methods:40 rats were randomly divided into four groups (10/group):group A(normal group), group B(Shenhuangcao-mixture to be convinced), group C(treated with coated oxidized starch) and group D(pathologic group). The later three groups were made to become acute renal injury models. Group A & group D used physiologic saline only. After ten days treatment, blood urea nitrogen(BUN), serum creatinine(SCr) and β-N-acetyglocosamidase (NAG) were monitored. And renal histological changes were observed. Results:In decreasing BUN, SCr & NAG, Shenhuangcao is superior to group C(P<0.01);It was also found that Shenhuangcao-mixture could reduce the edema of renal tubular by lightscope, the damage of mitochondria is lighter. Conclusion:Shenhuangcao-mixture can not only improve the renal function of the rats with damaged kidney, but also alleviate the edema and prevent the epithelial cells of the renal tubular from being damaged by gentamicin anymore.

关 键 词：急性肾损伤 庆大霉素 参黄草合剂 中药制剂 血肌酐 尿素氮 尿酶 肾小管上皮细胞 动物实验 

分 类 号：R277.592[医药卫生—中医学] R285.5