SDS、CTAB和PVP法提取香蕉基因组DNA的比较研究  

A comparative study of genomic DNAS from banana extracted by SDS, CTAB and PVP methods

作　　者：杜道林[1] 马文儒[1] 苏杰[1] 周鹏[2] 郑学勤[2] 

机构地区：[1]海南师范学院生物系,海南海口571158 [2]热带作物生物技术国家重点实验室,海南海口571101

出　　处：《海南师范学院学报（自然科学版）》2003年第1期74-80,共7页Journal of Hainan Normal University:Natural Science

基　　金：海南省教育厅项目(HJSK-1);农业部生物技术重点项目;海南省重点扶持学科"生态学";海南师范学院科研启动基金资助

摘　　要：文章报道了采用SDS法、CTAB法和PVP法提取的香蕉基因组DNA片段大小比较一致,都可直接用于RAPD分析,SDS法提取的DNA纯度较高,吸芽基因组DNA提取率略高于嫩叶,对相同clone不同单株间基因组DNA的RAPD分析结果并不表现出明显的多态性.The lengths of the genomic DNA fragments extracted by SDS, CTAB and PVP methods from banana are nearly alike and they all can be used in RAPD analysis, in which the DNA fragments extracted by SDS method is purer than those from the others. The DNA yield coming from buds is more than from young leaves. There is not any marked diversity among different plants of the same clone according to RAPD analysis.

关 键 词：香蕉 基因组DNA 提取方法 SDS法 CTAB法 PVP法 分子标记 基因多态性 比较研究 

分 类 号：S668.1[农业科学—果树学] Q781[农业科学—园艺学]