AFM观察小鼠心肌核DNA转录调控和蛋白质相互作用  

Observation of Transcription Regulation in the Mouse Heart Nuclear DNA Fragments and the Specific-protein Interaction by AFM

作　　者：袁明秀[1] 任重[1] 郑飞[1] 邓爱平[1] 李健伟[2] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]中国科学院化学研究所,北京100080

出　　处：《高技术通讯》2003年第4期34-38,共5页Chinese High Technology Letters

摘　　要：用原子力显微镜(AFM)直接观察、体外转录、PAGE电泳等多种实验技术组合,发现小白鼠(Balb/c)心肌核DNA片段中非转录状态的活性基因仅两端的调控序列结合支架蛋白(不可解离蛋白),同时非共价键特异结合自己编码的蛋白质与特定辅助信息小分子所形成的复合体(可解离蛋白)等多种转录调控蛋白质,中间的编码序列无结合蛋白质。转录状态的基因除两端的调控序列特异结合上述两类蛋白质外,中间的编码序列还以非共价键特异结合可完全解离的转录活性因子等多种蛋白质。本工作展示了未来运用AFM观察和体外转录等技术组合研究基因表达与调控机制的前景,并为阅读人类基因组图谱中确定某一特定基因的位置奠定了理论基础。Using atom force microscopy (AFM), in vitro transcription, PAGE and other experimental technologies, we observed that, in active genes of mice (Balb/c) nuclear DNA fragments of non-transcriptional state, only regulation sequences at both ends are associated with scaffold proteins (indissociable proteins) and some transcrip-tional factors such as complexes (dissociable proteins) made of gene-coding proteins and specific auxiliary small molecules, while there are no combining proteins in intermediate coding sequences. However, in active genes of transcriptional state, both regulation sequences and intermediate coding sequences are associated with active transcriptional factors by non-covalent bonds. Our work shows the prospective application of AFM observation and in vitro transcription in the research on gene expression and regulation. It also offers some theoretical basis for localization of specific genes in human genomes.

关 键 词：AFM 小鼠 心肌核 DNA 转录调控因子 分子生物学 蛋白质 相互作用 辅助信息小分子 NAD^+ 调节控制 基因表达 

分 类 号：Q752[生物学—分子生物学]