阳离子PLG纳米颗粒包裹对CpG序列免疫佐剂效应影响的研究  

The Effect of the Entrapment of CpG Sequence with Cationic PLG Nanoparticle on the Immune Responses of in Mice

作　　者：吕学斌[1] 李江凌[2] 高荣[1] 武梅[1] 吴凯源[1] 王丽焕[1] 沈翼[1] 刘崑[1] 郑勇[1] 刘世贵[1] 

机构地区：[1]四川大学生命科学学院草原生物防治工程国家专业实验室,成都610064 [2]四川省畜牧科学研究院,成都610066

出　　处：《高技术通讯》2003年第4期62-66,共5页Chinese High Technology Letters

基　　金：教育部资助项目。

摘　　要：本试验制备了聚乙交酯丙交酯(PLG)纳米颗粒,比较了阳离子PLG纳米颗粒和脂质体作为载体包裹CpG分子对小鼠体液和细胞免疫反应的影响。结果发现,阳离子PLG纳米颗粒包裹CpG能显著提高免疫小鼠总IgG,增加小鼠免疫细胞数量,增强淋巴细胞增殖活性及白细胞介素-2的诱生活性。它同阳离子脂质体作为包裹分子的效应相似或较强,能显著提高裸CpG质粒的免疫增强活性。Cationic PLG nanoparticles and cationic liposome were used as package molecules to detect the effect of CpG ODN on the cellular and humoral immune responses of mice. It was found that in comparison with the control groups, the number of immune cells, the amount of IgG, the induced bioactivity of IL-2 and proliferation of spleen lymphocytes were significantly increased in mice immunized with the cationic PLG nanoparticles with CpG. The stimulation of cationic PLG nanoparticles was similar to or stronger than that of cationic liposome. All these suggested that cationic PLG nanoparticle could be used as effective package molecule to raise the immunos-timulatory effect of CpGODN to animals.

关 键 词：聚乙交酯丙交酯 小鼠 体液 细胞免疫反应 阳离子PLG纳米颗粒 阳离子脂质体 CPG序列 免疫增强活性 免疫佐剂效应 传染性疾病防治 

分 类 号：R392[医药卫生—免疫学]