血小板粘合素α_2β_1基因多态性与2型糖尿病微血管病变相关性研究  

Study on the relationship between the platelet α_2β_1 integrin gene polymorphism and diabetic microangiopathy

作　　者：张军霞[1] 徐焱成[1] 朱宜莲[1] 范幼筠[1] 孙苏欣[1] 邓浩华[1] 

机构地区：[1]武汉大学中南医院内分泌科,430071

出　　处：《临床内科杂志》2003年第4期203-205,共3页Journal of Clinical Internal Medicine

摘　　要：目的　探讨血小板粘合素α2 β1分子α亚单位第 7内含子BglⅡ限制性片段多态性与2型糖尿病微血管并发症 (DMAP)的关系。方法　运用聚合酶链反应—限制性片段长度多态性技术 (PCR RFLP)检测 2 10例 2型糖尿病 (DM )患者的基因型 ,其中无微血管并发症 (NCD) 94例 ,糖尿病视网膜病变 (DR) 62例 ,糖尿病肾病 (DN) 5 4例 ,对照组 65例。结果　 ( 1)DR组BglⅡ ( +/+)基因型频率及BglⅡ ( +)等位基因频率明显高于NCD组及对照组 ,差异有显著性 ;( 2 )BglⅡ ( +/+)基因型频率及BglⅡ ( +)等位基因频率在DN组与NCD组之间及NCD组与对照组之间分布的差异均无显著性。结论　血小板粘合素α2 β1分子α亚单位遗传变异与DR发生相关 ,与DN及DM发生无关 ;BglⅡ ( +)等位基因可能是DR的易感基因 。Objective To evaluate the role of BglⅡ restriction fragment length polymorphism within intron 7 of the α sununit of platelet α 2β 1 integrin in Chinese type 2 diabetes mellitus(DM) with diabetic microangiopathy(DMAP).Methods 210 type 2 diabetic patients(94 without diabetic microangiopathy,DMAP group;62 patients with retinopathy,DR group;and 54 patients with nephropathy,DN group) and 65 normal subjects are recruited in the study.BglⅡ polymorphism is determinded by PCR RFLP. Results ①The frequencies of the BglⅡ(+/+) genotype and BglⅡ(+) allele in DR group are significantly higher than that in NCD group and control group;② The genotype frequency for BglⅡ(+/+) and the allele frequency for BglⅡ(+) are not significantly different between DN group and NCD group. It is same with NCD group and control group.Conclusions There is a strong correlation between the genetic variation in the α subunit within platelet α 2β 1 integrin and DR,but not DN.The allele BglⅡ(+) may be a susceptible gene of DR,but not contribute to DN.

关 键 词：2型糖尿病 微血管病变 血小板粘合素 α2β1基因 基因多态性 聚合酶链反应-限制性片段长度多态性技术 

分 类 号：R587.2[医药卫生—内分泌]