毛细管电泳免疫分析法分析雌三醇  

Determination of Estriol by Capillary Electrophoretic Immunoassay

作　　者：苏萍[1] 王永成[1] 张新祥[1] 常文保[1] 

机构地区：[1]北京大学化学学院化学生物学系,生物有机与分子工程教育部重点实验室,北京100871

出　　处：《分析化学》2003年第4期385-388,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .2 0 0 75 0 0 1;2 0 0 75 0 0 3)资助项目

摘　　要：利用一种温敏水凝胶作为毛细管电泳的填充介质 ,建立了毛细管电泳免疫分析血清中雌三醇的方法。研究了缓冲溶液的浓度和pH值、水凝胶的浓度、电压等因素对分析结果的影响。雌三醇的检出限和线性范围分别为 3 1 .6ng L和 5 0～ 5 0 0 0ng L。A kind of thermally linear polymer-poly-N-isopropylacrylamide (PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estroil antigen. A useful method for the determination of estroil in serum by capillary electrophoretic immunoassay was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage etc. has been studied. The limit of detection and the linear range of estriol were 31.6 ng/L and 50 similar to 5000 ng/L, respectively.

关 键 词：毛细管电泳免疫分析法 分析 雌三醇 温敏水凝胶 激光诱导荧光检测器 内源性激素 疾病 诊断 

分 类 号：O658.9[理学—分析化学]