马氏钳蝎蝎毒短肽BmK622的分离纯化和一级结构测定  

Purification and Primary Structure of a Toxin-like Peptide BmK622 from the Venom of Chinese Scorpion Buthus martensii Karsch

作　　者：张乃霞[1] 吴宫[1] 王跃丰[1] 王中华[1] 冯传莉[1] 吴厚铭[1] 

机构地区：[1]中国科学院上海有机化学研究所,上海200032

出　　处：《化学学报》2003年第4期630-634,共5页Acta Chimica Sinica

基　　金：国家自然科学基金 (No.2 0 1 32 0 30 )

摘　　要：应用凝胶过滤、离子交换和HPLC反相色谱法从马氏钳蝎粗毒中分离纯化得到蝎毒多肽BmK62 2 .联合运用串联质谱法和Edman降解法 ,鉴定了BmK62 2N端 19个残基的序列 ,经过数据库检索 ,发现数据库中用cDNA克隆方法鉴定了序列的马氏钳蝎蝎毒短肽BmTX3一级序列N端 19个残基与BmK62 2已测定的N端 19个残基序列完全相同 ,BmK62 2的分子量测定和氨基酸组成分析的结果表明 ,BmK62 2与BmTX3分子量相同、氨基酸组成一致 ,从而BmK62 2的一级结构为 :FGLIDVKCFASSECWTACKKVTGSGQGKCQNNQCRCY .The toxin-like peptide BmK622 has been purified from the venom of the Chinese scorpion Buthus martensii Karsch by using combined gel-filtration ion exchange and reversed phase chromatography techniques. The sequence of the peptide BmK622 was determined by the hybrid applying of tandem CID MS/MS, Edman degradation and database searching. Firstly the sequence for the N-terminal 19 amino acids-of BmK622 was obtained by using tandem CID MS/MS and Edman degradation. The following database searching revealed a hit. The sequence of N-terminal 19 amino acid residues of BmK622 is identical with that of the scorpion toxin BmTX3, which was determined by cDNA cloning. In addition, the molecular weight and the amino acid compositions of BmK622 are consistent with the calculated values according to them sequence of BmTX3. All these data suggested that BmK622 shared the same primary structure with BmTX3, and the intact sequence of BmK622 is as follows: FGLID VKCFA SSECW TACKK VTGSG QGKCQ NNQCR CY.

关 键 词：马氏钳蝎 蝎毒 短肽 BmK622 分离 纯化 串联质谱法 Edman降解法 一级结构 

分 类 号：O658[理学—分析化学]