聚丙烯微孔膜的等离子体改性及DNA原位合成  

Surface Modification of Polypropylene Microporous Membrane by Plasma and Its Application for the in situ Oligonucleotide Synthesis

作　　者：汤建新[1] 李松[1] 刘正春[1] 聂立波[1] 肖鹏峰[1] 陆祖宏[1] 何农跃[1] 

机构地区：[1]东南大学分子与生物分子电子学教育部重点实验室

出　　处：《化学学报》2003年第4期641-645,共5页Acta Chimica Sinica

基　　金：国家 8 63计划 (No.1 0 1 0 1 0 1 );973计划 (No.G1 9980 51 2 0 4 );国家自然科学基金 (No.60 0 71 0 0 1 );江苏省高新技术 (No.GB2 0 0 1 0 1 0 )

摘　　要：以H2 和N2 混合气体等离子体处理了聚丙烯微孔膜 ,采用扫描电镜观察了处理前后膜的形貌变化 ;真空全反射红外光谱和X射线光电子能谱证实在其表面接枝了大量活性氨基 ;用紫外光谱定量计算得知 ,所接枝的氨基浓度大约为 0 5μmol/cm2 .该改性膜可直接用于DNA的原位合成 ,其合成的DNA探针密度远高于功能化玻璃片基的探针密度 。Polypropylene microporous, membrane was treated with plasma in a mixture of nitrogen and hydrogen (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstrated the success of amino groups grafting. The density of active amino groups on the membrane surface is about 0.5 mumol/cm(2), which is much higher than that on modified glass slides. The as-handled membranes were successively applied to the in situ oligonucleotides synthesis and an average coupling yield, of more than 98% was achieved. These modified microporous membranes can be developed into a novel family of substrates for the in situ synthesis of DNA microarrays.

关 键 词：聚丙烯微孔膜 等离子体 改性 DNA 原位合成 接技 氨基 

分 类 号：O631[理学—高分子化学]