三唑磷杀虫剂的亚慢性毒性研究  

Study on Subchronic Toxicity of a Pesticide Triazophos in Rats

作　　者：董竞武[1] 肖萍[1] 郑志清[1] 俞筱琪[1] 叶秀玲[1] 施志冲[1] 符振华[1] 顾文华[1] 李真观[1] 

机构地区：[1]上海市疾病预防控制中心毒理室,上海200336

出　　处：《环境与职业医学》2003年第2期126-128,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的 ]研究三唑磷杀虫剂对大鼠的亚慢性毒性作用。 [方法 ]设 4组 ,每组 1 3只雌性和 1 3只雄性SD大鼠连续 1 3周分别以含三唑磷 0、1、2 0、40 0mg/kg摄食染毒 ,观察大鼠一般状况、体重及饲料、水消耗状况 ,并于第 7和 1 3周分别取大鼠的尿分析尿常规指标 ,于第 1 3周取大鼠的血分析血常规和生化指标 ;于染毒后第 2、4、6、1 3周分别取大鼠的血液分析其全血胆碱酯酶活性 ;于染毒后第 1 3周处死全部大鼠 ,取其脏器称重计算脏器系数 ,并通过病理组织检查分析对脏器的损害情况。 [结果 ]各剂量组大鼠 1 3周内均无死亡。40 0mg/kg剂量组大鼠的体重明显低于对照组 ,肝脏脏器系数以及血碱性磷酸酶、甘油三酯指标明显高于对照组。 2 0、40 0mg/kg组的红细胞数和血红蛋白数明显低于对照组。胆碱酯酶活性 4周时 40 0mg/kg剂量组雌鼠抑制率 37 1 % ,雄鼠抑制率 2 9 2 % ;2 0mg/kg剂量组雌鼠抑制率 1 5 7% ,雄鼠抑制率 1 3 2 % ;6周时 1mg/kg剂量组雌鼠抑制率 1 2 % ,雄鼠抑制率 6 9%。 [结论 ]大鼠 1 3周连续摄入 40 0mg/kg剂量的三唑磷对体重和肝脏有轻微影响。三唑磷最低观察到毒作用剂量为 2 0mg/kg,最大未观察到毒作用剂量为 1mg/kg。To study subchronic toxic effects of triazophos in rats. Four groups of 13 male and 13 female Sprague Dawley (SD) rats per each group were fed with feed stuff containing triazophos at 0,1,20 and 400 mg/kg feed stuff for 13 weeks. The general behaviour,body weight,feed stuff and water consumption were observed. The urine was collected at week 7 and 13 for urine routine testing. The blood was collected at week 13 for blood routine testing. At week 2,4,6 and 13 to measure the whole blood cholinesterase. All animals were sacrificed at week 13,the internal organs were taken to weigh and calculate the ratio of organ to body weight. The histopathological examimation was conducted. Survival was 100% during 13 weeks. The body weight of the rats in 400 mg/kg group was obviously lower than that in the control group. The liver to body weight ratio was obviously higher than that in the control group. The blood alkaline phosphatase(ALK) and triglycerides(Trig) were obviously higher than those in the control group. The red blood cell(RBC) number and hemoglobin(HGB) in 20 and 400 mg/kg groups were lower than those in the controls. At week 4, activity of whole blood cholinesterase was depressed in femals (37 1%) and in males (29 2%) in 400 mg/kg group,and also depressed in females (15 7%) and in males (13 2%) in 20 mg/kg group. At week 6,cholinesterese was also inhibited in females (12%) and in males (6 9%) in 1 mg/kg group. [Conclusion] There was slight effect on the body weight and liver of the rats administered orally 400 mg/kg feed stuff. The lowest observed effect level(LOEL) is 20 mg/kg. The no observed effect level(NOEL) is 1 mg/kg.

关 键 词：三唑磷杀虫剂 亚慢性毒性 血液分析 胆碱酯酶活性 病理组织检查 

分 类 号：R595.4[医药卫生—内科学]