γ-氨基丁酸受体γ_1亚基在大鼠前庭终器的表达  

Expression of γ-aminobutyric acid receptor γ_1 subunit in the end-organs of rat vestibule

作　　者：程华茂[1] 孔维佳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022

出　　处：《临床耳鼻咽喉科杂志》2003年第4期229-230,共2页Journal of Clinical Otorhinolaryngology

基　　金：国家自然科学基金(NO.39670781);卫生部优秀科技人才专项基金(NO.1995-144);教育部留学回国人员科技基金(NO.1996-644)

摘　　要：目的:研究γ-氨基丁酸受体γ_1亚基在大鼠前庭终器的表达。方法:运用RT-PCR技术检测大鼠前庭终器编码GABA_A受体亚基的基因表达。以大鼠脑组织RNA为阳性对照。结果:扩增出标志GABA_A受体基因表达的PCR产物。结论:GABA受体γ_1亚基在大鼠前庭终器有表达,为证明GABA是前庭系统重要神经递质之一提供了支持。Objective;To investigate the expression of the r-aminobutyric acid receptor r1 subunit in the end-organs of rat vestibule . Method;Using a combination of reverse transcription followed by polymerase chain reaction , expression of the y-aminobutyric acid receptor r1 subunit in the end-organs of rat vestibule was examined. The rat brain RNA was used as positive control . Result;PCR amplification products representing subunit gene expression for y-aminobutyric acid receptor a1 subunit were amplified. Conclusion: The results showed that y-ami-nobutyric acid receptor r1 subunit was expressed in the rat vestibule,indicating that GABA is one of the important neurotransmitters in the vestibular system.

关 键 词：前庭 Γ-氨基丁酸受体 逆转录-聚合酶链反应技术 大鼠 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]