以羊膜为载体培养角膜内皮细胞的实验研究  

Experimental study on amniotic membrane as the substrate for cultivating corneal endothelial cells in vitro

作　　者：傅瑶[1] 范先群[1] 刘伟[1] 商庆新[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院上海市组织工程研究重点实验室,200011

出　　处：《眼科研究》2003年第2期147-149,共3页Chinese Ophthalmic Research

基　　金：国家重点基础研究发展规划项目(973)组织工程的基本科学问题资助 (G1 9990 5430 9)

摘　　要：目的　以羊膜为载体培养角膜内皮细胞并形成单层组织 ,为以后内皮细胞层移植提供方法和材料来源。方法　胰酶消化去除羊膜上皮细胞 ,保留基底膜作为载体 ,种植兔角膜内皮细胞 ,体外培养形成单层内皮层 ,并进行形态、组织学、超微结构观察。结果　角膜内皮细胞在羊膜上黏附生长并增殖 ,体外培养 6～ 7d即融合成单层 ,其形态及生长密度优于单纯培养皿上培养的内皮细胞。复合角膜内皮组织由羊膜基底膜和单层扁平内皮细胞组成 ,与生理状态下的角膜内皮组织相近。结论　以羊膜为载体培养角膜内皮细胞可形成单层细胞层 。ObjectiveTo cultivate corneal endothelial cells on amniotic membrane in vitro in order to provide an effective stratagic method for clinical transplantation.MethodsCorneal endothelial cells were seeded on the basement membrane of amnion which had been prepared by removing the original epithelium.The composite corneal endothelium was then cultivated in vitro and its morphology,histology and ultrastructure were examined.ResultsCorneal endothelial cells could successfully proliferate on the amniotic membrane.After a culture period of one week,a monolayer of endothelial cells formed,which was found to resemble the normal tissues in morphology,histological structure and ultrastructure.ConclusionCorneal endothelium can be reconstructed in vitro by cultivating endothelial cells on amniotic membrane,which provides quality material for further transplantation.

关 键 词：角膜内皮细胞 羊膜 体外培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]