甲状腺激素自身抗体的检测及其临床意义  

The Determination of Thyroid Hormone Autoantibodies and Its Clinical Significance

作　　者：朱立[1] 连小兰[2] 苏薇[3] 郭芝生[2] 赵志英[1] 王正华[1] 白耀[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院核医学科,北京100730 [2]中国医学科学院中国协和医科大学北京协和医院内分泌科,北京100730 [3]中国医学科学院中国协和医科大学北京协和医院检验科,北京100730

出　　处：《标记免疫分析与临床》2003年第1期11-14,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：为探讨患者血清甲状腺激素水平假性升高的原因及检测甲状腺激素自身抗体的方法 ,为临床正确判断甲状腺激素水平提供帮助 ,本研究进行了甲状腺激素自身抗体结合实验、自身抗体稀释度实验、自身抗体抑制曲线实验及自身抗体检定实验。结果显示 :患者血清与12 5I -T3 或12 5I -T4呈特异性结合 ,结合率分别为 5 8.77%和 4 9.0 5 % ,是正常人或三蒸水对照的 8- 13倍 ;得到满意的T3 或T4自身抗体稀释度曲线和抑制曲线 ,并确定甲状腺自身抗体为IgG ,与兔抗人T3 或T4抗体出现在电泳同一位置。甲状腺激素水平假性升高的主要原因是患者体内存在着内源性抗甲状腺激素抗体 ,检测甲状腺激素自身抗体有利于正确判断甲状腺激素水平 。To study the reasons of falsely high concentrations of serum thyroid hormone and the way of determination of thyroid hormone autoantibodies, the experiments of thyroid hormone autoantibodies binding reaction, dilution testing, calibration curves and their check analysis were performed. Results showed that the combination of the autoantibodies with 125 I-T 3 or 125 I-T 4 was specific, the binding rates were 58.77% and 49.05% respectively and 7-12 times higher than control groups. The radioactive peaks of the autoantibodies and rabbit anti-T 3 or T 4 antibody appeared on the same position in radio electrophoretogram analysis and these antibodies were considered as IgG. The important reasons of falsely high concentrations of serum thyroid hormone are the presence of anti-thyroid hormone antibodies. Determination of thyroid hormone autoantibodies significantly benefits diagnosis and treatment of thyroid disease.

关 键 词：甲状腺激素 甲状腺激素自身抗体 血清检测 

分 类 号：R446.62[医药卫生—诊断学]